Alanine-Scanning of the 50's Loop in the Clostridium beijerinckii Flavodoxin: Evaluation of Additivity and the Importance of Interactions Provided by the Main Chain in the Modulation of the Oxidation-Reduction Potentials

摘要

The four-residue reverse turn-Met~56-Gly-Asp-Glu~59-in the Clostridium beijerinckii flavodoxin provides the majority of the critical interactions with the isoalloxazine ring of the flavin mononucleotide (FMN) cofactor that contribute to the binding and the differential stabilization of its three redox states.