Structures of the Michaelis Complex (1.2 angstrom) and the Covalent Acyl Intermediate (2.0 angstrom) of Cefamandole Bound in the Active Sites of the Mycobacterium tuberculosis beta-Lactamase K73A and E166A Mutants

摘要

The genome of Mycobacterium tuberculosis (TB) contains a gene that encodes a highly active beta-lactamase, BlaC, that imparts TB with resistance to beta-lactam chemotherapy. The structure of covalent BlaC-beta-lactam complexes suggests that active site residues K73 and E166 are essential for acylation and deacylation, respectively. We have prepared the K73A and E166A mutant forms of BlaC and have determined the structures of the Michaelis complex of cefamandole and the covalently bound acyl intermediate of cefamandole at resolutions of 1.2 and 2.0 angstrom, respectively. These structures provide insight into the details of the catalytic mechanism.