Modification of cytotoxic effects, due to variable-rate heating, by changing osmotic pressure in the medium or by chloramphenicol application

摘要

We investigated the influence of media with different osmotic pressure and of chloramphenicol on cytotoxic effects of heating with different rate of Escherichia coli B/r and Escherichia coli B_(5-1) bacteria, and Zygosaccharomyces bailii yeast cells. It was shown that the hypotonic media appreciably increased cytotoxic action of heating with different rate, and, on the contrary, the hypertonic media inhibited induction of these effects. The inhibitor of protein synthesis chloramphenicol was established not to affect the bacterial thermoresistance in the process of different heating rate. On the basis of analysis of the obtained and literature data, it is proposed that a reason of cell injury dependence of the heating rate may be the availability of dissimilar levels of osmotic homeostasis destabilization in these cells when heated with different rates.