Water purification, recycle and improvement eco-system on polluted water area using effective organisms and materials

摘要

The aim of this research is to develop in situ water treatment systems producing useful materials. Porous ceramics for the carrier of microorganism and the purification of water were prepared from waste materials such as waste ceramics, volcanic ash and oyster shells. The immobility of microorganisms on ceramic carriers was evaluated by the DNA measurement. Much amount of microorganisms was immobilized on ceramics carrier having pores of dozens μm in diameter. Continuous production of Poly-b-Hydroxybutyric Acid (PHD) by microorganisms accumulated from river water was achieved with the perfusion culture system using a shaken ceramic membrane flask (SCMF). The PHD content became 35 wt% after 140 h of culture time in the single SCMF system was employed by feeding the propanol medium. On the other hand, the two-stage perfusion culture method using twin SCMFs was employed to increase seed cell mass in the first SCMF, which was supplied for producing PHD in the second flask. Consequently, PHD content increased up to 51 wt% in the second SCMF. These results demonstrated that the two-stage perfusion culture system was useful for producing PHD accompanied with the wastewater treatment.