Fused Core Particles for HPLC Columns

摘要

In recent years, there has been a strong movement toward the use of HPLC columns with smaller particles. While columns with 5-um particles have long been the standard, some users now have turned to columns of 3.5 μm or smaller2 to produce faster separations and increase sample throughput. The advantages of smaller particles are well known: faster separations without sacrificing resolution so that more samples can be analyzed in the same time period. Of course, there are always tradeoffs that must be made to gain this speed. Smaller particles decrease the permeability of columns; thus increased pressures must be used, resulting in increased stress on pumping systems and other instrument components.