Determination of the bactericidal activity of blood serum by recombinant luminescence strains of Escherichia coli

摘要

It has been experimentally proven as possible to use recombinant strains of Escherichia coli carrying the genes of luminescence of natural fluorescent bacteria in evaluating the bactericidal activity of blood serum (BABS). The shaping fluorescence inhibition was shown to be in proportion to the developing bactericidal effect in respect to the luminescent strains of Escherichia coli, which depended on a used concentration of blood serum and time of contact. The list of E. coli recombinant strains, which fit in most of all with the evaluation of BABS, was drawn. The values of the bactericidal activity of blood serum determined by the routine bacteriological and nephelometric methods were demonstrated to coincide with those determined by the worked-out bio-luminescent method.