Genetic diversity and phylogenetic relationships of the genus Lycopersicon (Tourn.) Mill. as revealed by inter-simple sequence repeat (ISSR) analysis

摘要

A protocol was developed to study the genetic diversity and phylogenetic relationships in 54 wild accessions and cultivars of the genus Lycopersicon using inter-simple sequence repeat (ISSR) analysis. Some 318 ISSR fragments were amplified for the wild and cultivated tomato genomes. The ISSR primers revealed an interspecific polymorphism of 95.6%. Species-specific ISSR fragments were detected for each tomato species. The highest number (more than 20) of species-specific fragments were obtained forL. esculentum sensu lato, although the intraspecific variation of ISSR patterns was low. UPGMA cluster analysis was used to construct a dendrogram and to estimate the genetic distances between species of Lycopersicon; between populations of L. peruvianum, L. pimpinellifolium, and L. esculentum; and between tomato cultivars.