Soluble and Immobilized Phenol Oxidase of the Fungus Mycelia sterilia IBR 35219/2: A Comparative Study

摘要

Phenol oxidase (EC1.14.18.1) from the microscopic fungus Mycelia sterilia IBR 35219/2 was immobilized using glutaraldehyde on macroporous silica carriers. The enzyme immobilized on amino-Silochrome SKh-2 or aminopropyl-Silochrome 350/80 exhibited maximum activity. Soluble and immobilized phenol oxidases were compared. Compared to the soluble enzyme, the activity of which was optimum at pH5.5, immobilized phenol oxidase exhibited optimum activity under slightly more acidic conditions (pH5.2). Immobilization considerably increased the enzyme stability. Both soluble and immobilized forms of phenol oxidase from M.sterilia IBR 35219/2 catalyze oxidative conversion of phenolic compounds of the green tea extract.