Quantitative detection of methylated SOCS-1,a tumor suppressor gene,by a modified protocol of quantitative real time methylation-specific PCR using SYBR green and its use in early gastric cancer detection

摘要

Although methylation-specific PCR (MSP) is a sensitive technique in the detection of DNA hypermethylation,it is not quantitative.Here we described a modified PCR protocol to quantify methylated SOCS-1 gene by real time MSP using SYBR green,which involves an additional PCR step after the 72 °C extension step.This modified protocol is also useful in the quantitative detection of methylated SOCS-1 gene in serum samples of gastric cancer patients.