Cl- currents activated via purinergic receptors in Xenopus follicles.

摘要

Ionic currents elicited via purinergic receptors located in the membrane of Xenopus follicles were studied using electrophysiological techniques. Follicles responded to ATP-activating inward currents with a fast time course (F(in)). In Ringer solution, reversal potential (Erev) of F(in) was -22 mV, which did not change with external substitutions of Na- or K+, whereas solutions containing 50 or 5% of normal Cl- concentration shifted Erev to about +4 and +60 mV, respectively, and decreased F(in) amplitude, indicating that F(in) was carried by Cl-.F(in) had an onset delay of approximately 400 ms, measured by application of a brief jet of ATP from a micropipette positioned near the follicle (50 microns). F(in) was inhibited by 50% in follicles pretreated with pertussis toxin. This suggests a G protein-mediated receptor channel pathway. F(in) was mimicked by 2-MeSATP and UTP, the potency order (half-maximal effective concentration) was 2-MeSATP (194 nM) > UTP (454 nM) > ATP (1,086 nM). All agonists generated Cl- currents and displayed cross-inhibition on the others. F(in) activation by acetylcholine also cross-inhibited F(in)-ATP responses, suggesting that all act on a common channel-activation pathway.