A Simple Approach to Patterned Protein Immobilization on Silicon via Electrografting from Diazonium Salt Solutions

摘要

A highly versatile method utilizing diazonium salt chemistry has been developed for the fabrication of protein arrays. Conventional ultraviolet mask lithography was used to pattern micrometer sized regions into a commercial photoresist on a highly doped p-type silicon (100) substrate. These patterned regions were used as a template for the electrochemical grafting of the in situ generated p-arninobenzenediazonium cation to form patterns of aminophenyi film on silicon. Immobilization of biomolecules was demonstrated by coupling biotin to the aminophenyi regions followed by reaction with fluorescently labeled avidin and visualization with fluorescence microscopy. This simple patterning strategy is promising for future application in biosensor devices.