Inactivation of dry endotoxin by several sterilization methods

摘要

The method to recover dry endotoxin from glassware and the effect of several sterilization methods on the inactivation of dry endotoxin were examined by using Limulus gelation activity of endotoxin activity. The following results were obtained, 1. Dry endotoxin was effectively recovered from glassware by mixing with glass beads in the water. 2. About 70～90% of endotoxin activity was lost by any of treatment with gamma irradiation, electron beam irradiation, ethylene oxide gas, moist heat, hydrogen peroxide gas plasma and hydrogen peroxide/peracetic acid gas plasma. The residual activity was not further inactivated by any of the sterilization methods tested even in stronger conditions.