Efficient propagation method of tara vine (Actinidia arguta Planch.) by etiolation culture.

摘要

Nodal segments of current shoots of A. arguta were cultured (primary culture) and the nodal segments with one lateral bud from the primary culture were transferred and subcultured (secondary culture) repeatedly on Miller medium containing 0, 1 or 10鎊250LM NAA and/or benzyladenine (BA) at 25鳦 under light or dark conditions. In primary culture, the rate of shoot formation increased with the addition of BA and decreased at the high NAA concentration; shoot elongation was promoted by the combination of BA and NAA and the number of lateral buds per shoot was largest on medium containing 1 鍹 NAA + 10 鍹 BA. Roots formed in 100% of cultures on media containing NAA. In secondary culture, shoot formation was suppressed by the high NAA concentration, but waspromoted by the addition of BA. In the dark, etiolated shoots with long internodes grew more rapidly and formed more lateral buds than those grown under light. When etiolated shoots were subcultured in the dark, shoot length, internode length and numberof lateral buds increased more promptly than in light cultures. When the propagating-culture procedure was repeated with a unit culture duration of 15, 20 or 25 days in darkness, shoot length and number of lateral buds on etiolated shoots increased significantly with unit culture duration, but did not vary after the 3rd subculture among the 3 treatments. Mathematical relationships between variables and results were derived.