The exposure to trans-cinnamic acid of osmotically stressed Catharanthus roseus cells cultured in a 14-l bioreactor increases alkaloid accumulation

摘要

A Catharanthus roseus cell line was cultured in a 14-l bioreactor. Total alkaloid production decreased more than 80% while scaling up this cell line from 250 ml batch cultures to the bioreactor. However, the subsequent application of an osmotic stress and 1 mM trans-cinnamic acid, which inhibits the synthesis of phenolic compounds, restored the original alkaloid amounts.