Metabolomic analysis of heat-hardening in adult green-lipped mussel (Perna canaliculus): A key role for succinic acid and the GABAergic synapse pathway

摘要

We evaluated the thermotolerance (LT50) of adult green-lipped mussels (Perna canaliculus) following an acute thermal challenge in the summer of 2012 and the winter of 2013. Mussels were grouped into two treatments, naive (N, no prior heat treatment) and heat-hardened (HH = 1 h at 29 degrees C, 12 h recovery at ambient) before being immersed for 3 h in water of varying temperature, i.e. Ambient (Control), 25, 29, 31, 33, and 35 degrees C with subsequent mortality monitored for 30 days. As expected, naive mussels were less thermotolerant than heat-hardened i.e. Summer LT50, N = 31.9, HH = 33.5 degrees C; Winter LT50, N = 31.4, HH = 33.8 degrees C. Moreover, at 33 degrees C no heat-hardened mussels died compared to 100% mortality in naive specimens. At 35 degrees C all mussels died regardless of treatment.