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首页> 外文期刊>Journal of vascular research >Regional differences in platelet-derived growth factor production by the canine aorta.
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Regional differences in platelet-derived growth factor production by the canine aorta.

机译:犬系主动脉血小板衍生生长因子生产的区域差异。

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摘要

Platelet-derived growth factor (PDGF) is a potent mitogen and chemotactic agent which may be involved in the formation of proliferative lesions of the arterial system, such as intimal hyperplasia and atherosclerosis. To examine the regional variation in vessel wall production of this mitogen, PDGF production and PDGF A chain mRNA expression by normal arterial wall was studied as a function of vessel location. PDGF production by canine aortic segments was measured after 72 h in organ culture, revealing significantly more PDGF produced by the distal compared to proximal aorta at 77 +/- 10 versus 14 +/- 6 pg/cm2/72 h (p<0.05). Endothelial cells (EC) and smooth muscle cells (SMC), isolated from analogous aortic sites, were grown in tissue culture and the conditioned medium was assayed for PDGF. EC in vitro demonstrated a similar geographic trend in PDGF production (distal=1,501 +/- 389 pg/microgram DNA/72 h, proximal=759 +/- 230 pg/microgram DNA/72 h; p=0.17). PDGF production by SMC in cell culture had a similar pattern with cells from the distal aorta producing 58 +/- 28 pg PDGF/microgram DNA/72 h, compared to cells from the proximal aorta producing 37 +/- 15 pg PDGF/microgram DNA/72 h (p=0.13). Freshly harvested EC and SMC, isolated from the same aortic sites, were subjected to quantitation of PDGF mRNA levels using a coupled reverse transcriptase and polymerase chain reaction amplification method, with glyceraldehyde-phosphate dehydrogenase (GAPDH) as a control. The ratio of PDGF A chain:GAPDH mRNA was significantly greater in distal aortic SMC, 2.30 +/- 0.99, compared to proximal aortic SMC, 1.27 +/- 0.46 (p=0.05), but was not significantly different between proximal and distal aortic EC (p=0.86). These findings demonstrate significant regional differences in PDGF production in the normal canine aorta. Additionally, SMC are implicated as a significant contributor to the regional variation in PDGF production.
机译:血小板衍生的生长因子(PDGF)是一种有效的丝分裂性和趋化剂,其可参与形成动脉系统的增殖性病变,例如内膜增生和动脉粥样硬化。为了检查该丝裂原,PDGF生产和PDGF的血管壁产生的区域变化,作为血管位置的函数研究了正常动脉壁的链条mRNA表达。在器官培养物中72小时后测量犬主动脉段的PDGF产生,与近端主动脉为77 +/- 10对14 +/- 6 pg / cm2 / 72h(P <0.05)相比,通过远端产生的PDGF明显更多的PDGF(P <0.05) 。与类似主动脉位点分离的内皮细胞(EC)和平滑肌细胞(SMC)在组织培养中生长,测定条件培养基的PDGF。 EC体外展示了PDGF生产的类似地理趋势(远端= 1,501 +/- 389页/微克DNA / 72h,Proximal = 759 +/- 230 pg / micrograch DNA / 72h; p = 0.17)。 SMC在细胞培养中产生的PDGF产生与来自远端主动脉的远端主动脉的细胞具有类似的图案,与来自近端主动脉的细胞产生37 +/- 15 pg PDGF / Micrograph DNA的细胞相比/ 72 h(p = 0.13)。使用偶联的逆转录酶和聚合酶链反应扩增法与糖绿醛 - 磷酸脱氢酶(GAPDH)为对照进行新鲜收获的EC和SMC。与近端主动脉SMC相比,PDGF A链:GAPDH mRNA的比率显着更大,2.30 +/- 0.99,1.27 +/- 0.46(P = 0.05),但在近端和远端主动脉之间没有显着差异EC(P = 0.86)。这些研究结果表明了正常犬系主动脉中PDGF产生的显着区域差异。此外,SMC涉及到PDGF生产区域变异的重要因素。

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