Determination of Congo red in food samples by methyl-beta-cyclodextrin/Triton X-100 synergistic sensitized fluorescence quenching method of the derivatives of calix[4]arene

摘要

A novel fluorescent chemosensor of the derivatives of calix[4]arene (SAX) for Congo red (CR) in methyl-beta-cyclodextrin/Triton X-100 synergistic sensitized system was developed. The results showed that the fluorescence intensity of SAX could be quenched by CR, and the fluorescence quenching (a dagger F = F (SAX) - F (CR-SAX)) was synergistic sensitized by methyl-beta-CD and Triton X-100. Under the conditions of lambda (ex/em) = 332/468 nm and pH 7.5, the linear range and the detection limit for CR were found to be 0.040-8.0 mu g/mL and 8.9 ng/mL, respectively. The mechanism of determination was discussed by quenching type analysis and sensitizing effect. This method has also been applied for the determination of CR in beef samples with satisfactory results.