Visualizing the interaction of <fc xmlns='http://www.wiley.com/namespaces/wiley'> <fi>Acanthamoeba castellanii</fi> </fc> <fi xmlns='http://www.wiley.com/namespaces/wiley'>Acanthamoeba castellanii</fi>Acanthamoeba castellanii with human retinal epithelial cells by spontaneous Raman and CARS imaging

Naemat Abida; Sinjab Faris; McDonald Alison; Downes Andy; Elfick Alistair; Elsheikha Hany M.; Notingher Ioan

摘要

Abstract > Improved understanding of the mechanism of nutrient's uptake can enable targeted manipulation of nutrient sensing pathways in medically important pathogens to a greater degree than is currently possible. In this context, we present the use of spontaneous Raman microspectroscopy and coherent anti‐Stokes Raman spectroscopy to visualize the time‐dependent molecular interactions between the protozoan <fc> <fi>Acanthamoeba castellanii</fi> </fc> and host human cells. Human retinal pigment epithelial (ARPE‐19) cells were pre‐labelled with deuterated Phe (L‐Phe[D8]) and the uptake of the host derived L‐Phe(D8) by <fc> <fi>A.?castellanii</fi> </fc> trophozoites was measured by Raman microspectroscopy for up to 48?hr post infection (hpi). This approach revealed a time‐dependent uptake pattern of this essential amino acid by <fc> <fi>A.?castellanii</fi> </fc> trophozoites during the first 24 hpi with complete enrichment with L‐Phe(D8) detected in trophozoites at 48 hpi. In contrast, cell free <fc> <fi>A.?castellanii</fi> </fc> trophozoites showed a modest uptake of only 16–18% L‐Phe(D8) from L‐Phe(D8)–supplemented culture medium after 3, 24, and 48?hr hpi. Coherent anti‐Stokes Raman spectroscopy microscopy was successfully used to monitor the reprogramming of lipids within the trophozoites as they engaged with host cells. The methodology presented here provides new advances in the ability to analyze the kinetic of amino acid acquisition by <fc> <fi>A.?castellanii</fi> </fc> from host cell and extracellular environment, and to visualize lipid reprogramming within the trophozoite. </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><abstract xmlns =“http://www.wiley.com/namespaces/wiley”type =“main”xml：lang =“en”> <title type =“main”>抽象</ title> >改进了解营养素的摄取机制可以在医学上的重要病原体中进行营养传感途径的靶向操纵，比目前可能的程度更大。在这种情况下，我们介绍了自发拉曼微斑镜检查和相干的抗刺激拉曼光谱，以可视化原生动物<Fc> <Fi> Acanthamoba Castellanii </ Fi> </ Fc>和宿主人细胞之间的时间依赖性分子相互作用。用氘化PHE（L-PHE [D8]）预先标记人视网膜颜料上皮（ARPE-19）细胞，并通过<FC> <FI> A.?castellanii <co. / fi> </ fc>通过拉曼微穴位测量滋磨剂，最多48个？HR后感染（HPI）。该方法揭示了该必需氨基酸的时间依赖于该必需氨基酸的上注模式<FC> <FI> A.Castellanii <Castellanii </ Fi> </ Fi>滋养体，其在前24 HPI期间具有完全富集的L-PHE（D8）在48 HPI的滋养体中。相比之下，无细胞<Fc> <FI> A.Castellanii </ Fi> </ FC>滋养本质表明，来自L-PHE（D8）的培养物的仅16-18％L-PHE（D8）的适度摄取3,24和48次后的中等HPI。连贯的抗斯托克斯拉曼光谱显微镜显微镜被成功地用于监测与宿主细胞接通的滋养体内脂质的重编程。这里呈现的方法提供了通过来自宿主细胞和细胞外环境的<FC> <FI> A.CASTELLANII </ FI> </ FI> </ FI> </ FI> </ FI> </ FI>，以及在内部显示脂质重编程的能力的新进展滋养体。 </ p> </ abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-35645/'>《Journal of Raman Spectroscopy: An International Journal for Original Work in All Aspects of Raman Spectroscopy, Including Higher Order Processes, and Also Brillouin- and Rayleigh Scattering》</a> <b style="margin: 0 2px;">|</b><span>2018年第3期</span><b style="margin: 0 2px;">|</b><span>共12页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Naemat Abida&option=202" target="_blank" rel="nofollow">Naemat Abida;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Sinjab Faris&option=202" target="_blank" rel="nofollow">Sinjab Faris;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=McDonald Alison&option=202" target="_blank" rel="nofollow">McDonald Alison;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Downes Andy&option=202" target="_blank" rel="nofollow">Downes Andy;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Elfick Alistair&option=202" target="_blank" rel="nofollow">Elfick Alistair;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Elsheikha Hany M.&option=202" target="_blank" rel="nofollow">Elsheikha Hany M.;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Notingher Ioan&option=202" target="_blank" rel="nofollow">Notingher Ioan;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>School of Physics and AstronomyUniversity of NottinghamNottingham NG7 2RD UK;</p> <p>School of Physics and AstronomyUniversity of NottinghamNottingham NG7 2RD UK;</p> <p>School of Engineering Institute for BioEngineeringUniversity of Edinburgh The King's BuildingsMayfield Road Edinburgh EH9 3DW UK;</p> <p>School of Engineering Institute for BioEngineeringUniversity of Edinburgh The King's BuildingsMayfield Road Edinburgh EH9 3DW UK;</p> <p>School of Engineering Institute for BioEngineeringUniversity of Edinburgh The King's BuildingsMayfield Road Edinburgh EH9 3DW UK;</p> <p>School of Veterinary Medicine and ScienceUniversity of NottinghamCollege Road Leicestershire LE12 5RD UK;</p> <p>School of Physics and AstronomyUniversity of NottinghamNottingham NG7 2RD UK;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/4724.html" title="光谱学">光谱学;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Acanthamoeba castellanii&option=203" rel="nofollow">Acanthamoeba castellanii;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Coherent anti‐Stokes Raman scattering&option=203" rel="nofollow">Coherent anti‐Stokes Raman scattering;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Host‐pathogen interaction&option=203" rel="nofollow">Host‐pathogen interaction;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Isotope labelling&option=203" rel="nofollow">Isotope labelling;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Raman Spectroscopy&option=203" rel="nofollow">Raman Spectroscopy;</a> </p> <div class="translation"> 机译：Acanthamoeba Castellanii;连贯的反斯托克斯拉曼散射;宿主病原体相互作用;同位素标记;拉曼光谱; </div> </li> </ul> </div> </div> <div class="literature cardcommon"> <div class="similarity "> <h3 class="all_title" id="enpatent66">相似文献</h3> <div class="similaritytab clearfix"> <ul> <li class="active" >外文文献</li> </ul> </div> <div class="similarity_details"> <ul > <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704025815075.html">Visualizing the interaction of <fc xmlns="http://www.wiley.com/namespaces/wiley"> <fi>Acanthamoeba castellanii</fi> </fc> <fi xmlns="http://www.wiley.com/namespaces/wiley">Acanthamoeba castellanii</fi>Acanthamoeba castellanii with human retinal epithelial cells by spontaneous Raman and CARS imaging</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Naemat Abida&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Naemat Abida,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Sinjab Faris&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Sinjab Faris,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=McDonald Alison&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">McDonald Alison,</a> <a href="/journal-foreign-35645/" target="_blank" rel="nofollow" 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Visualizing the interaction of Acanthamoeba castellanii Acanthamoeba castellaniiAcanthamoeba castellanii with human retinal epithelial cells by spontaneous Raman and CARS imaging

摘要