Peroxiredoxin-2 and zinc-alpha-2-glycoprotein as potentially combined novel salivary biomarkers for early detection of oral squamous cell carcinoma using proteomic approaches

摘要

Abstract No effective screening method is available for oral squamous cell carcinoma (OSCC) that is recognized to influence by environmental factors as well as human papillomavirus (HPV) and Epstein-Barr virus (EBV). Therefore, we sought to identify salivary biomarkers for screening of OSCC with or without HPV and/or EBV infection. Saliva, lesion and oral exfoliated cells were collected from OSCC patients and cancer-free controls (CFCs) and grouped depending on their HPV- and EBV-infection status. Salivary protein was precipitated and subjected to 2-dimensional gel electrophoresis. Differential expression of proteins was identified by mass spectrometry and validated by Western blotting. Distinctive expression patterns of salivary proteins were detected in OSCC as compared with CFCs. Levels of peroxiredoxin-2 (PRDX-2) and zinc-alpha-2-glycoprotein (ZAG) were significantly up-regulated in OSCC cases (p Significance Our findings demonstrate a useful of combined detection of PRDX-2 and ZAG as a salivary biomarker for the early detection of OSCC. Graphical abstract Display Omitted Highlights ? 7 proteins that differentially expressed were identified using mass spectrometry. ? PRDX-2, ZAG, HP were significantly up-regulated in saliva of OSCC cases. ? PRDX-2 and ZAG were also up-regulated in lesion of OSCC cases. ? PRDX-2 and ZAG yielded a highest discriminatory power in distinguishing OSCC patients from CFCs. ? Combination of salivary PRDX-2 and ZAG could be useful as the biomarker for OSCC.