A simple and highly sensitive UPLC-ESI-MS/MS method for the simultaneous quantification of nicotine, cotinine, and the tobacco-specific carcinogens N’-nitrosonornicotine and 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone in serum samples

摘要

Highlights?Quantification of nicotine, cotinine, NNN and NNK in serum poses many challenges.?Current methods ofen rely on complex multi-step procedures.?One-pot liquid-liquid extraction procedure using only 10μL of serum was developed.?HILIC chromatography enables direct injection of the extract onto the LCMS system.?Excellent sensitivity, accuracy and precision of the method are demonstrated.AbstractAccording to the World Health Organization, the consumption of tobacco products is the single largest cause of preventable deaths in the world, exceeding the total aggregated number of deaths caused by diseases such as AIDS, tuberculosis, and malaria. An important element in the evaluation of the health risks associated with the consumption of tobacco products is the assessment of the internal expo