One‐step immunochromatographic visual assay for anti‐transglutaminase detection in organ culture system: An easy and prompt method to simplify the in vitro diagnosis of celiac disease

摘要

Background Anti‐tissue transglutaminase (anti‐ tTG ) and endomysium antibodies ( EMA ) are detectable in duodenal culture media of celiac disease ( CD ) patients. To improve the management of this organ culture system, we evaluated the anti‐ tTG occurrence by immunochromatographic assay ( ICA ). Methods A total of 103 CD patients and 41 disease controls underwent duodenal biopsy for the organ culture. In culture supernatants, IgA anti‐ tTG were tested by both enzyme‐linked immunosorbent assay ( ELISA ) and ICA , IgA EMA were searched by indirect immunofluorescence analysis ( iIFA ). Results Endomysium antibodies and anti‐ tTG measured by ELISA were positive in culture media of all CD patients, while anti‐ tTG detected by ICA were positive in culture media of 87/103 CD patients. Anti‐ tTG ICA scores significantly correlated with anti‐ tTG ELISA values ( r =.71, P .0001). Sensitivity, specificity and diagnostic accuracy of anti‐ tTG detected by ICA were 84.5%, 100% and 88.9%, respectively. Conclusions Using ICA , anti‐ tTG are detectable in duodenal culture media of most CD patients and the intensity of indicative lines depends on the anti‐ tTG concentration. Sensitivity and diagnostic accuracy achieved with ICA are lower than those obtained with ELISA but, given that the first is a more easy and prompt method, data suggest the possibility of utilizing it in the in vitro diagnosis of CD.