Biosynthesis of Silver Nanoparticles Using Fenugreek Seed Extract and Evaluation of Their Antifungal and Antibacterial Activities

摘要

Synthesizing nanoparticles by biological methods using microorganisms, plants parts, plant extracts and enzymes have attracted great attention recently because of their cost-effective, eco-friendly and nontoxic methods. In this research, silver nanoparticles (AgNPs) were synthesised from silver nitrate (AgN0_3) solution by green synthesis process with the assistance of fenugreek seed extract. The detailed characterization of the AgNPs were carried out using UV-Visible spectroscopy, transmission electron microscopy (TEM) and Fourier transform infrared (FTIR) spectroscopy. Zetasizer was determined an average size of the resulting nanoparticles. Antimicrobial and antifungal assays of synthesized AgNPs were also carried out against bacterial and fungal cells respectively. The results showed significant bactericidal activity against K. pneumoniae, B. subtilis and S. aureuswhile Escherichia coli was least inhibited. Amongst the fungi screened both F. equiseti and A. alternata showed maximum inhibition.