Production of butyric acid by        <fi xmlns='http://www.wiley.com/namespaces/wiley'>Clostridium tyrobutyricum</fi>Clostridium tyrobutyricum        coated with carboxyl functioned magnetic nanoparticles

Huang Jin; Tang Wan; Zhu Shengquan; Du Meini

摘要

Abstract <section xml:id="jctb5582-sec-0001" numbered="no"> <title type="main">BACKGROUND > Magnetic nanomaterial has been considered a promising carrier for cell immobilization, because of its low mass transfer resistance, high load rate, high specific surface and facility for reusability. In this study, carboxyl functioned magnetic nanoparticles (CMNPs) were prepared by chemical co‐precipitation and oleate modification methods, and then immobilized with <fi>Clostridium tyrobutyricum</fi> for the production of butyric acid. </section> <section xml:id="jctb5582-sec-0002" numbered="no"> <title type="main">RESULTS > The immobilization conditions were systematically optimized to achieve maximum efficiency and the highest cell relative activity, in which the mass ratio, immobilization temperature and time were determined as 0.72 g g ‐1 , 35 °C and 30 min, respectively. The endurance of CMNPs‐cells was also evaluated in a repeated‐batch mode of eight continuous batches. Stable and reliable production of butyric acid was obtained in each batch with an average butyrate titer and yield of ～11.02 g L ‐1 and ～0.43 g g ‐1 utilized sugar. Compared with the free‐cell fermentation, higher butyrate yield and concentration were obtained with immobilized cells of <fi>C. tyrobutyricum</fi> in repeated‐batch fermentation, suggesting that the coated cells did not experience a mass transfer problem. </section> <section xml:id="jctb5582-sec-0003" numbered="no"> <title type="main">CONCLUSION >Considering the convenient separation of CMNPs‐cells from fermen </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><Abstract XMLNS =“http://www.wiley.com/namespaces/wiley”type =“main”xml：lang =“en”> <title type =“main”>抽象</ title> <section XML：ID =“JCTB5582-SEC-0001”编号=“否”> <标题类型=“main”>背景</标题> >磁性纳米材料已被认为是用于细胞固定的有功率的载体，因为其低传质阻力，高负荷率，高比表面和可重用性的设施。在该研究中，通过化学共沉淀和油eate改性方法制备羧基功能型磁性纳米颗粒（CMNP），然后用<Fi>梭菌酪蛋白粘蛋白为固定化，用于生产丁酸的制备。 </ p> </ section> <section XML：ID =“JCTB5582-SEC-0002”编号=“否”> <标题类型=“main”>结果</ title> >固定条件系统地优化实现最大效率和最高细胞相对活性，其中质量比，固定温度和时间分别测定为0.72gg -1 / sop>，35℃和30分钟。还以八个连续批次的重复批量模式评估CMNPS细胞的耐久性。在每间批次中获得稳定可靠的丁酸生产，平均丁酸滴度和产率〜11.02g L -1 -1和〜0.43g g -1 / sup>利用糖。与自由细胞发酵相比，用固定化的<FI> C的细胞获得更高的丁酸盐产率和浓度。在重复分批发酵中的酪乳酪乳酪乳酪蛋白</ fi>，表明涂层细胞没有经历传质问题。 </ p> <section XML：ID =“JCTB5582-SEC-0003”编号=“否”> <标题类型=“main”>结论</ title> >，考虑方便的CMNPS分离 - 来自Fermen的细胞 </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-33908/'>《Journal of Chemical Technology & Biotechnology》</a> <b style="margin: 0 2px;">|</b><span>2018年第8期</span><b style="margin: 0 2px;">|</b><span>共7页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Huang Jin&option=202" target="_blank" rel="nofollow">Huang Jin;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Tang Wan&option=202" target="_blank" rel="nofollow">Tang Wan;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Zhu Shengquan&option=202" target="_blank" rel="nofollow">Zhu Shengquan;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Du Meini&option=202" target="_blank" rel="nofollow">Du Meini;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>College of Pharmaceutical ScienceZhejiang University of TechnologyHangzhou China;</p> <p>College of Pharmaceutical ScienceZhejiang University of TechnologyHangzhou China;</p> <p>College of Pharmaceutical ScienceZhejiang University of TechnologyHangzhou China;</p> <p>College of Pharmaceutical ScienceZhejiang University of TechnologyHangzhou China;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/180.html" title="生物工程学（生物技术）">生物工程学（生物技术）;</a><a href="https://www.zhangqiaokeyan.com/clc/20827.html" title="生物化学过程">生物化学过程;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Clostridium tyrobutyricum&option=203" rel="nofollow">Clostridium tyrobutyricum;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=carboxyl functioned magnetic nanoparticles&option=203" rel="nofollow">carboxyl functioned magnetic nanoparticles;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=immobilization&option=203" rel="nofollow">immobilization;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=butyric acid&option=203" rel="nofollow">butyric acid;</a> </p> <div class="translation"> 机译：Tyrobuticum粉末;羧基功能磁性纳米颗粒;固定化;丁酸; </div> </li> </ul> </div> </div> <div class="literature cardcommon"> <div class="similarity "> <h3 class="all_title" id="enpatent66">相似文献</h3> <div class="similaritytab clearfix"> <ul> <li class="active" >外文文献</li> <li >专利</li> </ul> </div> <div class="similarity_details"> <ul > <li> <div> <b>1. </b><a class="enjiyixqcontent" 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Production of butyric acid by Clostridium tyrobutyricumClostridium tyrobutyricum coated with carboxyl functioned magnetic nanoparticles

摘要