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Factors affecting the selection of PHB accumulating methanotrophs from waste activated sludge while utilizing ammonium as their nitrogen source

机译:影响废物活性污泥中PHB积累甲胰酶的因素,同时利用铵作为氮源

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Abstract <section xml:id="jctb5502-sec-0001" numbered="no"> <title type="main">BACKGROUND >Methanotrophs can offer a long‐term reliable solution to two of the major problems causing environmental pollution. These microorganisms can convert methane, i.e. second major greenhouse gas (GHG), into biodegradable polymers as polyhydroxybutyrate (PHB). In this research, waste activated sludge was used as a seed for cultivating enrichments utilizing methane and ammonium as their sole carbon and nitrogen sources respectively with high PHB accumulation capacity. Moreover, to test the effect of different parameters, such as the initial ratio between the ammonium and the microorganisms (N/M), food to microorganisms ratio (F/M), the solids retention time (SRT), copper elimination and nitrate, on the selection of PHB accumulating, i.e. Type II methanotrophs. </section> <section xml:id="jctb5502-sec-0002" numbered="no"> <title type="main">RESULTS > After establishing a mixed culture dominated by Type II methanotrophs, the enriched culture was kept in the exponential phase of growth and had a stable performance for almost 30 consecutive cycles with a specific growth rate of 0.077 ± 0.005 h ‐1 and biomass yield of 0.81 ± 0.06 mg‐VSS mg ‐1 ‐CH <sub>4</sub> . The PHB accumulation capacity of the enrichment reached 52.9 ± 4% with a yield of 0.54 ± 0.12 mg‐PHB mg ‐1 ‐CH <sub>4</sub> . </section> <section xml:id="jctb5502-sec-0003" numbered="no"> <title type="main">CONCLUSIONS >Increasing the N/M ratio can be employed to ensure the dominance of Type II methanotrophs in mixed c </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译:</span><Abstract XMLNS =“http://www.wiley.com/namespaces/wiley”type =“main”xml:lang =“en”> <title type =“main”>抽象</ title> <section XML:ID =“JCTB5502-SEC-0001”编号=“否”> <标题类型=“main”>背景</ title> >甲蛋白可以为两个主要问题提供长期可靠的解决方案,导致环境污染的两个主要问题。这些微生物可以将甲烷,即第二主要温室气体(GHG)转化为可生物降解的聚合物,为多羟基丁酸酯(PHB)。在该研究中,废物活化污泥分别用作培养富含甲烷和铵作为其唯一碳和氮源的富集的种子,并具有高pHB累积能力。此外,为了测试不同参数的效果,例如铵和微生物(n / m)之间的初始比率(n / m),食物对微生物比(f / m),固体保留时间(srt),铜消除和硝酸盐,在累积pHB的选择中,即II型甲蛋白。</ p> </截面> <部分XML:ID =“JCTB5502-SEC-0002”编号=“否”> <标题类型=“main”>结果</标题> >在建立由II型甲蛋白的甲胰剂中占据的混合培养物后,将富集的培养物保持在展望的生长阶段,并且具有稳定的性能,几乎具有0.077±0.005 h 的特定生长速率-1 </ sup>和生物量产率为0.81±0.06mg-Vss mg -1 </ sup> -ch <sub> 4 </ sub>。富集的PHB累积能力达到52.9±4%,产率为0.54±0.12mg-PHB mg -1 </ sup> -ch <sub> 4 </ sub>。 </ p> </ section> <section XML:ID =“JCTB5502-SEC-0003”编号=“否”> <标题类型=“main”>结论</ title> >增加N / M比率可以用来确保混合C中II型甲蛋白的优势 </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-33908/'>《Journal of Chemical Technology & Biotechnology》</a> <b style="margin: 0 2px;">|</b><span>2018年第5期</span><b style="margin: 0 2px;">|</b><span>共11页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Fergala Ahmed&option=202" target="_blank" rel="nofollow">Fergala Ahmed;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=AlSayed Ahmed&option=202" target="_blank" rel="nofollow">AlSayed Ahmed;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Eldyasti Ahmed&option=202" target="_blank" rel="nofollow">Eldyasti Ahmed;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>Department of Civil EngineeringYork UniversityToronto Ontario Canada;</p> <p>Department of Civil EngineeringYork UniversityToronto Ontario Canada;</p> <p>Department of Civil EngineeringYork UniversityToronto Ontario Canada;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/180.html" title="生物工程学(生物技术)">生物工程学(生物技术);</a><a href="https://www.zhangqiaokeyan.com/clc/20827.html" title="生物化学过程">生物化学过程;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=methanotrophs&option=203" rel="nofollow">methanotrophs;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=activated sludge&option=203" rel="nofollow">activated sludge;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=biogas&option=203" rel="nofollow">biogas;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=food to microorganisms ratio&option=203" rel="nofollow">food to microorganisms ratio;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=solids retention time&option=203" rel="nofollow">solids retention time;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=PHB&option=203" rel="nofollow">PHB;</a> </p> <div class="translation"> 机译:甲胰蛋白;活性污泥;沼气;食物到微生物比例;固体保留时间;PHB; 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href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=符茹&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 符茹</a> <span> . 2011</span> </span> </div> </li> </ul> <ul style="display: none;"> <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/patent-detail/061201655914.html">一种利用联碱法生产长效氯化铵为氮源的长效缓释复混肥料及制备方法</a> <b>[P]</b> . <span> 中国专利: CN103073366B </span> <span> . 2015.06.03</span> </div> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/patent-detail/06120101706719.html">一种利用联碱法生产长效氯化铵为氮源的长效缓释复混肥料及制备方法</a> <b>[P]</b> . <span> 中国专利: CN103073366A </span> <span> . 2013-05-01</span> </div> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/patent-detail/06130415865881.html">Intermittent application of reduced nitrogen sources for selection of PHB producing methanotrophs</a> <b>[P]</b> . <span> 外国专利: <!-- 美国专利: --> US2015159185A1 </span> <span> . 2015-06-11</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译:间歇性使用还原氮源选择生产PHB的甲烷营养生物 </span> </p> </li> <li> <div> <b>4. </b><a class="enjiyixqcontent" href="/patent-detail/06130417402220.html">INTERMITTENT APPLICATION OF REDUCED NITROGEN SOURCES FOR SELECTION OF PHB PRODUCING METHANOTROPHS</a> <b>[P]</b> . <span> 外国专利: <!-- 世界知识产权组织专利: --> WO2013181225A1 </span> <span> . 2013-12-05</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译:减少氮源的间歇性应用在生产PHB的甲醇中的选择 </span> </p> </li> <li> <div> <b>5. </b><a class="enjiyixqcontent" href="/patent-detail/06130421936097.html">Manufacturing Process of Activated Sludge for Partial Nitrification of Ammonium-ion Compound and Waste-water Treatment Process Including Ammonia or Ammonium-ion Compound Using the Activated Sludge</a> <b>[P]</b> . <span> 外国专利: <!-- 韩国专利: --> 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