Preparation of Multi-Scale Hydrogel Scaffolds for Tissue Engineering Through Biologic Hydrogel 3D Printing and Forming System

摘要

In the past decades, significant progress has been made in the field of tissue engineering, especially the development of hydrogel scaffold. The scaffold that has microchannels plays an important role in tissue vascularization, which is useful to repair the defected tissues and organs. However, the existing hydrogel scaffold has some drawbacks, such as unstable structure and poor mechanical properties. Therefore, it is necessary to develop a more flexible scaffold preparation method. In this paper, we propose a novel Biologic Hydrogel 3D Printing and Forming System based on ion cross-linking molding (sodium alginate and calcium chloride), by precisely controlling the printing process, multi-scale scaffolds with hollow and solid structure can be obtained. The preparation process of the scaffold was integrated, which improves both performance and efficiency. Compression experiments showed that the multi-scale scaffolds possess better mechanical properties, and in vitro experiments demonstrated that the fabrication process is biocompatible with cells.