Characterization of 27 novel microsatellite loci in Sinibotia superciliaris (Gunther 1892) and cross-species transferability in Sinibotia reevesae (Chang 1944)

摘要

The Chinese golden loach, Sinibotia superciliaris and its congener Sinibotia reevesae, are endemic to China and extraordinary similar in morphological traits. However, few genetic studies have been conducted on them, especially those based on nuclear markers. Here, we developed 27 novel polymorphic microsatellite markers from S.superciliaris and further tested the cross-species transferability of those loci in S.reevesae. The cross-species amplification test showed that 19 loci demonstrate polymorphism in S.reevesae. The mean number of alleles (N-A) were both 3 in S.superciliaris and S.reevesae, the mean expected heterozygosities (H-E) were 0.57 and 0.54, the Shannon-Wiener Diversity Indices (SW) were 0.84 and 0.82, and the evenness (E) were 0.09 and 0.08, respectively. The polymorphic information content (PIC) showed a high level of polymorphism for the two species (mean 0.54 and 0.50, respectively). In addition, the cross-species transferability (100%) of those markers was clearly high, which confirmed that the microsatellite markers developed here could be used effectively for other related species.