Histidine Tagged Protein Recovery from Tobacco Extract by Foam Fractionation

摘要

Tobacco plants have the potential to be used for the production of proteins for pharmaceutical applications.This work describes a novel protein recovery strategy where the protein of interest is "tagged" with a histidine sequence,which forms a complex with cobalt ions and surfactant possessing a chelating functionality such that the protein is recovered in the foamate of a foam fractionation step His-gus,a histidine-tagged enzyme,was chosen as a model protein to study the feasibility of this strategy.The His-gus is recovered from spiked prefoamed tobacco etract by foam fractionation in the presence of surfactant and cobalt ions with an enrichment of 1.29 and a recovery of 21.5% in terms of an adjusted activity.