Protocols for Husbandry and Embryo Collection of a Parthenogenetic Gecko, Lepidodactylus lugubris (Squamata: Gekkonidae)

摘要

Lizards and snakes (squamate reptiles) have become increasingly used in developmental biology research, resulting in the establishment of several "model" lizard clades or species (e.g., Sanger et al. 2008; McLean and Vickaryous 2011; Diaz et al. 2017; Infante et al. 2018; Londono et al. 2017; Sanger and Kircher 2017). When choosing a species or clade to study for developmental questions, several criteria must be met. First, a species or group of species must be identified which exhibit the genotype or phenotype of interest. Second, practical criteria must also be considered. The species or group of species must be available for experimentation or observation in the laboratory. Preferably, the species would be available to purchase or easy to obtain from wild populations, easily housed in a laboratory setting with standardized husbandry protocols, have a high fecundity, and have additional resources for investigating developmental questions, such as a sequenced genome or transcriptomes.