Development of a bacterial consortium comprising oil-degraders and diazotrophic bacteria for elimination of exogenous nitrogen requirement in bioremediation of diesel-contaminated soil

摘要

The purpose of this study was to develop an effective bacterial consortium and determine their ability to overcome nitrogen limitation for the enhanced remediation of diesel-contaminated soils. Towards this, various bacterial consortia were constructed using oil-degrading and nitrogen-fixing microbes. The diesel removal efficiency of various developed consortia was evaluated by delivering the bacterial consortia to the diesel-contaminated soils. The consortium Acinetobacter sp. K-6+Rhodococcus sp. Y2-2+NH4NO3 resulted in the highest removal (85.3%) of diesel from the contaminated soil. The consortium containing two different oil-degrading microbes (K-6+Y2-2) and one nitrogen-fixing microbe Azotobactervinelandii KCTC 2426 removed 83.1% of the diesel from the soil after 40days of treatment. The total nitrogen content analysis revealed higher amounts of nitrogen in soil treated with the nitrogen-fixing microbe when compared with that of the soil supplemented with exogenous inorganic nitrogen. The findings in this present study reveal that the consortium containing the nitrogen-fixing microbe degraded similar amounts of diesel to that degraded by the consortium supplemented with exogenous inorganic nitrogen. This suggests that the developed consortium K-6+Y2-2+KCTC 2426 compensated for the nitrogen limitation and eliminated the need for exogenous nitrogen in bioremediation of diesel-contaminated soils.