Shoot-Tip Culture of Acerola (Malpighia glabra L.)

摘要

Culture conditions for shoot tips excised from cutting propagated plants of aceraia (Malpighia glabra L.) were investigated to develop a shoot-tip culture system. Shoot tips of aceraia "Sanmikei (Hosoba) " were grown on a medium, consisting of MS basal components with 3% sucrose. The addition of 0.1 ppm 6-benzylaminopurin (BA), 0.05 ppm BA + 0.05 ppm zeatin or 0.1 ppm BA + 0.1 ppm zeatin was effective for the proliferation of shoots. Those that had grown two to three cm from the subculture, were examined further for rooting, which had been stimulated when the shoots were immersed in 250 ppm indole-3-butyric acid (IBA) for 15 seconds. Ten to eleven days of dark treatment enhanced this. All plantlets were recovered after acclimatization. Sequence-related amplified polymorphism (SRAP) was conducted to validate the genetic homogeneity of the regenerated plants. In SRAP analysis, using 13 primer combinations, no polymorphisms among the regenerated and original plants were detected.