A 37 kDa Txp40 protein characterized from Photorhabdus luminescens sub sp akhurstii conferred injectable and oral toxicity to greater wax moth, Galleria mellonella

摘要

Photorhabdus luminescens is a gram-negative bacterium that symbiotically associates with insect-parasitic nematode, Heterorhabditis indica. Herein, we have characterized an insecticidal gene, Txp40 (1008 bp) from the indigenous isolates of P. luminescens, and tested its bioefficacy against Galleria mellonella via injectable and oral bioassay. The recombinant protein characterized from P. luminescens strain H3 exhibited comparatively greater insect toxicity than strain H1 in terms of LD50 and LT50 values. Txp40 holds great potential to replace Bt toxins in global agriculture.