首页> 外文期刊>The journals of gerontology.Series A. Biological sciences and medical sciences >Comprehensive miRNA Profiling of Skeletal Muscle and Serum in Induced and Normal Mouse Muscle Atrophy During Aging
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Comprehensive miRNA Profiling of Skeletal Muscle and Serum in Induced and Normal Mouse Muscle Atrophy During Aging

机译:老化诱导和正常小鼠肌肉萎缩骨骼肌综合分析

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Age-associated loss of muscle mass and function is a major cause of morbidity and mortality in the elderly adults. Muscular atrophy can also be induced by disuse associated with long-term bed rest or disease. Although miRNAs regulate muscle growth, regeneration, and aging, their potential role in acute muscle atrophy is poorly understood. Furthermore, alterations in circulating miRNA levels have been shown to occur during aging but their potential as noninvasive biomarkers for muscle atrophy remains largely unexplored. Here, we report comprehensive miRNA expression profiles by miRNA-seq analysis in tibialis anterior muscle and serum of a disuse-induced atrophy mouse model, mimicking the acute atrophy following long-term bed rest, as compared to those of young and old mice. Comparative analysis and validation studies have revealed that miR-455-3p was significantly decreased in muscle of both induced-atrophy model and old mice, whereas miR-434-3p was decreased in both serum and muscle of old mice, as compared to young mice. Furthermore, upregulation of miR-455-3p in fully differentiated C2C12 myoblasts induced a hypertrophic phenotype. These results suggest that deregulation of miR-455-3p may play a functional role in muscle atrophy and miR-434-3p could be a candidate serum biomarker of muscle aging.
机译:年龄相关的肌肉质量丧失和功能是老年人发病率和死亡率的主要原因。肌肉萎缩也可以通过与长期卧床休息或疾病相关的废物诱导。虽然miRNA调节肌肉生长,再生和衰老,但它们在急性肌肉萎缩中的潜在作用被理解得很差。此外,已经显示出在老化期间发生循环miRNA水平的改变,但它们作为肌肉萎缩的非侵入性生物标志物的潜力仍然很大程度上是未开发的。在这里,我们通过MiRNA-SEQ分析在胫骨前肌肉和血清中报告综合的miRNA表达谱,与年轻老鼠相比,模拟了长期卧床休息后的急性萎缩。比较分析和验证研究表明,诱导萎缩模型和旧小鼠的肌肉中miR-455-3p显着降低,而MiR-434-3P在旧小鼠的血清和肌肉中减少,与年轻小鼠相比。此外,在完全分化的C2C12肌细胞中的miR-455-3p的上调诱导肥厚表型。这些结果表明miR-455-3p的放松管制可能在肌肉萎缩中发挥功能性作用,MiR-434-3p可以是肌肉衰老的候选血清生物标志物。

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