An improved micropropagation protocol for the recalcitrant plant Capsicum - a study with ten cultivars of Capsicum spp. (C-annuum, C-chinense, and C-frutescens) collected from diverse geographical regions of India and Mexico

摘要

Capsicum spp. is a commercially important crop of the Solanaceae family, well-known for its multipurpose use as a vegetable, spice, medicinal and ornamental plants. The genus Capsicum is a recalcitrant species in terms of in vitro morphogenesis and plant regeneration. An efficient method was developed for multiple shoot regeneration in 10 cultivars of Capsicum collected from diverse geographical regions of India and Mexico. Seeds germinated in vitro on a half-strength Murashige and Skoog (MS) medium supplemented with 3.0 % sucrose. Nodes of the in vitro germinated seedlings were used as explant for micropropagation. The combination of the 6-benzylaminopurine, indole-3-acetic acid, and spermidine was found to be the best for multiple shoot induction. However, the optimum responcse varied accompanied by different cultivers with maximum 8.9 +/- 0.52 (Capsi-10) to 15.3 +/- 0.69 (Capsi-5) multiple shoot per explant. Depending on the cultivar, multiplied shoots were successfully rooted with maximum 18.4 +/- 0.20 (highest for Capsi-9) to 36.8 +/- 0.29 (highest for Capsi-5) roots per shoot on half-strength MS medium supplemented with 2.0 mg l(-1) indole-3-butyric acid, 1.0 mg l(-1) alpha-naphthalene acetic acid, and 1.5 mM spermidine. Finally, the micropropagated plantlets were acclimatized with 40.0-86.7 % survival rate, depending on different cultivars.