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首页> 外文期刊>The journal of gene medicine >In vitroIn vitro and in vivoin vivo gene delivery using chitosan/hyaluronic acid nanoparticles: Influences of molecular mass of hyaluronic acid and lyophilization on transfection efficiency
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In vitroIn vitro and in vivoin vivo gene delivery using chitosan/hyaluronic acid nanoparticles: Influences of molecular mass of hyaluronic acid and lyophilization on transfection efficiency

机译：体外和在体外和中 使用壳聚糖/透明质酸纳米粒子的体内基因递送的体内递送：透明质酸分子量和冻干对转染效率的影响

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Abstract <section xml:id="jgm2968-sec-0001" numbered="no"> <title type="main">Background > Lyophilization is an effective method for preserving nonviral gene vectors. To improve the stability and transgene expression of lyophilized plasmid DNA (pDNA) complexes, we coated the surfaces of pDNA/chitosan complexes with hyaluronic acid (HA) of varying molecular masses. The transgene expression of pDNA/chitosan/HA ternary complexes was characterized in vitro and in vivo . </section> <section xml:id="jgm2968-sec-0002" numbered="no"> <title type="main">Methods >pDNA complexes were lyophilized overnight and the resultant products with spongy, porous consistencies were stored at ?30, 4 or 25°C for 2?weeks. Rehydrated complexes were characterized using gel retardation assays, aiming to confirm complex formation, measure particle size and evaluate zeta potential, as well as conduct luciferase gene reporter assays. The anti‐tumor effects of pDNA ternary complexes were evaluated using suicide gene (pTK) coding thymidine kinase in Huh7‐implanted mice. </section> <section xml:id="jgm2968-sec-0003" numbered="no"> <title type="main">Results >Transfection efficiencies of pDNA/chitosan/HA ternary complexes were dependent on the average molecular masses of HA. The coating of pDNA/chitosan complexes with HA maintained the cellular transfection efficiencies of lyophilized pDNA ternary complexes. Furthermore, intratumoral injection of lyophilized, rehydrated pDNA ternary complexes into tumor‐bearing mice showed a significant suppression of tumor growth. </section> <section xml:id="jgm2968-sec-0004" numbered="no"> <title type="main">Conclusions </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><abstract xmlns =“http://www.wiley.com/namespaces/wiley”type =“main”> <title type =“main”>抽象</ title> <section XML：ID =“JGM2968-SEC-0001 “编号=”否“> <标题类型=”main“>背景</标题> >冻干是保留非血流基因载体的有效方法。为了改善冻干质粒DNA（PDNA）复合物的稳定性和转基因表达，我们用不同分子量的透明质酸（HA）涂覆了PDNA /壳聚糖络合物的表面。 PDNA /壳聚糖/ HA三元复合物的转基因表达在体外，体内的体外</ i>。 </ p> </ section> <部分XML：ID =“JGM2968-SEC-0002”编号=“否”> <标题类型=“main”>方法</标题> > PDNA复合物被冻干过夜和带有海绵状，多孔浓度的所得产品储存在α30,4或25℃下2个？周。使用凝胶延迟测定表征再水化复合物，旨在确认复杂的形成，测量粒度和评估Zeta电位，以及进行荧光素酶基因报告分析。使用自杀基因（PTK）编码胸苷激酶评估PDNA三元复合物的抗肿瘤作用。</ p> </段> <截面XML：ID =“JGM2968-SEC-0003”编号=“否“> <标题类型=”main“>结果</标题> > PDNA /壳聚糖/ HA三元复合物的转染效率取决于HA的平均分子量。具有HA的PDNA /壳聚糖复合物的涂层保持冻干PDNA三元复合物的细胞转染效率。此外，孵化的冻干的冻干的PDNA三元络合物进入肿瘤的小鼠的抑制性抑制肿瘤生长。</ p> </段> <截面XML：ID =“JGM2968-SEC-0004”编号=“否” > <标题类型=“main”>结论</ title> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-30882/'>《The journal of gene medicine》</a> <b style="margin: 0 2px;">|</b><span>2017年第8期</span><b style="margin: 0 2px;">|</b><span>共1页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Sato Toshinori&option=202" target="_blank" rel="nofollow">Sato Toshinori;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Nakata Mitsuhiro&option=202" target="_blank" rel="nofollow">Nakata Mitsuhiro;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Yang Zhihong&option=202" target="_blank" rel="nofollow">Yang Zhihong;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Torizuka Yu&option=202" target="_blank" rel="nofollow">Torizuka Yu;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Kishimoto Satoko&option=202" target="_blank" rel="nofollow">Kishimoto Satoko;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Ishihara Masayuki&option=202" target="_blank" rel="nofollow">Ishihara Masayuki;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>Department of Biosciences and InformaticsKeio UniversityYokohama Kanagawa Japan;</p> <p>Department of Biosciences and InformaticsKeio UniversityYokohama Kanagawa Japan;</p> <p>Department of Biosciences and InformaticsKeio UniversityYokohama Kanagawa Japan;</p> <p>Department of Biosciences and InformaticsKeio UniversityYokohama Kanagawa Japan;</p> <p>Research Support CenterDokkyo Medical UniversityShimotsuga Tochigi Japan;</p> <p>Research Institute National Defense Medical CollegeTokorozawa Saitama Japan;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/1473.html" title="医学遗传学">医学遗传学;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=anti‐tumor effect&option=203" rel="nofollow">anti‐tumor effect;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=chitosan&option=203" rel="nofollow">chitosan;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=gene transfer&option=203" rel="nofollow">gene transfer;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=hyaluronic acid&option=203" rel="nofollow">hyaluronic acid;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=lyophilization&option=203" rel="nofollow">lyophilization;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=suicide gene therapy&option=203" rel="nofollow">suicide gene therapy;</a> </p> <div class="translation"> 机译：抗肿瘤作用;壳聚糖;基因转移;透明质酸;冻干;自杀基因治疗; </div> </li> </ul> </div> </div> <div class="literature cardcommon"> <div class="similarity "> <h3 class="all_title" id="enpatent66">相似文献</h3> <div class="similaritytab clearfix"> <ul> <li class="active" >外文文献</li> <li >专利</li> </ul> </div> <div class="similarity_details"> <ul > <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704024826607.html"><i xmlns="http://www.wiley.com/namespaces/wiley">In vitroIn vitro and <i xmlns="http://www.wiley.com/namespaces/wiley">in vivoin vivo gene delivery using chitosan/hyaluronic acid nanoparticles: Influences of molecular mass of hyaluronic acid and lyophilization on transfection efficiency</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Sato Toshinori&option=202" target="_blank" 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