Growth performance and gastrointestinal responses in heavy Tom turkeys fed antibiotic free corn-soybean meal diets supplemented with multiple doses of a single strain Bacillus subtilis probiotic (DSM29784)

摘要

Growth performance and gastrointestinal (GIT) responses to a single-strain of Bacillus subtilis (SSB) were investigated using 960 Hybrid Converter Toms. A total of 4 iso-caloric and iso-nitrogenous corn-soybean meal-based diets were allocated to 12 replicate cages/pens and fed (ad libitum) in a four-phase feeding program (starter; days 0 to 28, grower-1; days 29 to 56, grower-2; days 57 to 84, and finisher; days 85 to 126). The diets had either 0 (control), 1E+08 (low), 2E+08 (mid) or 1E+09 (high) cfu B. subtilis/kg. Feed intake (FI) and BW were recorded by phase. Excreta samples were collected towards the end of starter and grower-1 phases for apparent retention (AR) of components by marker method and litter moisture, respectively. Selected birds were necropsied on days 28 and 56 for GIT weight and samples for jejunal histomorphology and ceca digesta short chain fatty acids (SCFA). Supplemental SSB had linear and non-linear (P 0.05) response on BW gain (BWG). Specifically, relative to the control, birds fed low SSB had higher BWG in starter and grower-1 phases whereas bird fed mid and high SSB had lower BWG in grower-2 and birds fed low and mid SSB had higher BWG in finisher phase. Consequently, birds fed low and mid SSB doses were heavier (P 0.05) than control fed birds at the end of trial. The FCR response to SSB was linear and non-linear (P 0.05) with birds fed low SSB showing lower FCR than control fed birds in starter, grower-1 and finisher phases. Supplemental SSB had linear and non-linear (P 0.05) effects on AR of components (DM, ash, crude protein, crude fat, neutral detergent fiber, and gross energy), litter moisture, GIT weight, jejunal histomorphology, and SCFA. Relative to control, birds fed high SSB showed higher AR of components, villi height, day 56 ceca digesta total SCFA concentration, and lower litter moisture. In conclusions, under condition of the current study, growth performance was optimized by low to mid SSB. Improved nutrient retention and indices of gut health suggested higher SSB doses may optimize growth performance under challenging farm conditions.