Colonization of internal organs by Salmonella Enteritidis in experimentally infected laying hens of four commercial genetic lines in conventional cages and enriched colony housing

摘要

The prevalence of Salmonella Enteritidis in commercial egg-laying flocks is a prominent public health concern because contaminated eggs cause human illness. Deposition of this pathogen inside eggs results from bacterial colonization of reproductive tissues in infected hens. Environmental conditions can influence avian Salmonella infections, but the food safety consequences of different poultry housing systems remain uncertain. The present study assessed the invasion of internal organs by Salmonella Enteritidis in groups of experimentally infected laying hens of four commercial genetic lines (designated as white egg lines W1 and W2 and brown egg lines B1 and B2). Groups of hens from each line were housed at 555 cm(2) of floor space per bird in both conventional cages and colony units enriched with access to perches and nesting areas. All hens were orally inoculated with 5.75 x 10(7) colony-forming units of a two-strain Salmonella Enteritidis mixture. At 6 to 7 d post-inoculation, hens were euthanized, and samples of liver, spleen, ovary, oviduct, and intestinal tract were removed for bacteriologic culturing. The frequency of Salmonella Enteritidis recovery from intestinal samples was significantly (P 0.05) greater for the two white egg lines combined than for the two brown egg lines combined in both conventional cage (72.2% vs. 50.0%) and enriched colony housing systems (66.7% vs. 37.5%). The frequency of intestinal Salmonella Enteritidis isolation from line B1 was significantly higher from hens in conventional cages (47.2%) than in enriched colonies (22.2%), but no differences were observed for other hen lines. Line W1 yielded more positive intestinal samples than either brown egg line in conventional cages, and line B2 had fewer positive intestinal samples than all other lines in enriched colonies. There were no significant differences between hen lines or housing systems in Salmonella Enteritidis isolation from other internal organs. These results demonstrate that Salmonella Enteritidis colonization of the intestinal tract can vary between genetic lines of egg-laying hens and that some lines are subject to housing system influences on Salmonella susceptibility.