Evaluation of metabolic changes in oxalate-rich plant Rumex obtusifolius L. caused by ion beam irradiation

摘要

AbstractSomeRumexspecies such as sorrel are edible as baby leaf salad greens. On the other hand,Rumexplants accumulate soluble oxalate, a toxic metabolite which causes serious diseases such as renal syndrome. We attempted to produce low-oxalate plants ofR. obtusifolius, a perennial weed which has higher vitamin C and amino acid content and higher tolerance to stress than many otherRumexspecies. Ion beams are ionising radiation with high linear energy transfer that causes a wide spectrum of mutations. Thus, in the present study we evaluated the effects of ion beams on oxalate and other primary metabolites in leaves ofR. obtusifoliususing CE-MS. The results showed that oxalate content was increased by irradiation with carbon ion beams. Metabolome analysis revealed that ion beams affected carbon flow to the isocitrate pathway, which is involved in oxalate synthesis. These observations suggested that modulation of carbon flow to the isocitrate pathway is important to regulate oxalate levels in plants.Highlights?The ion beam-irradiation on seeds enhanced oxalate accumulation in leaves.