The effects of mutation and temperature variation on monacolin K production by monascus sp. and relative statistical parameter analysis of monacolin K production

摘要

Monacolin K is a secondary metabolite of the Monascus species that restrains cholesterol synthesis. Temperature control is an effective method of improving the rate of monacolin K synthesis. Ultraviolet (UV) irradiation and ethylmethane sulfonate (EMS) were employed to mutate the Monascus strain so that it could be studied. After mutation by UV and EMS, the best monacolin K production strain was scanned after the 2-h UV irradiation treatment. Monascus U-2 is defined as Monascus after UV irradiation for 2 h and generates the maximum production of monacolin K production. In the primary incubation period, monacolin K production of this strain was exceptionally low. Afterwards, cell growth reached the maximum value of 1.1 g/L after incubation for seven days. During the incubation process, the maximum production of monacolin K reached 8437.6 mg/kg by Monascus U-2, and the red pigment was 11.52. In summary, this study shows that the mutated strain U-2 can produce more monacolin K than the mutated strain U-10 and U-EMS.