Collection of Drosophila suzukii Pupae from Wheat Germ Diet, by Water Washing

摘要

To optimize Drosophila suzukii rearing and establish mass rearing its local parasitoids, this washing method collects pupae from wheat germ diet. The diet consists of purified water (480 ml), raw wheat germ (100 g), table sugar (45 g), Brewers yeast (35 g), and maize flour (30 g). Preparation requires stirring and boiling all ingredients for 7 minutes, then cooling to room temperature. Implementation of the method allows collecting clean pupae and compiling them into a smaller space, so it helps mass rearing D. suzukii and its parasitoids. Additionally, it could facilitate execution of other ideas with potential to control the pest, among them, the sterile insect technique and sterile insect technique + entomopathogenic fungi.