Lipid extraction and production of biodiesel from marine diatom Chaetoceros muelleri isolated from Vellar estuary, southeast coast of India

摘要

The marine algae based biodiesel may be the greatest possible options for future energy, particularly for India where land resources and freshwater are limited. In the above context, The aim of this research is to reveal the informtion on growth and lipid content of Chaetoceros muelleri'm different medium varying Si concentration, isolated from Vellar estuary, South east coast of India and characterized at different Si treatment as lipid production. In this experiment, C. muelleri showed maximum celldensity in H^day in treamentT4 (Si concentration 159 uM) 19.11 ±0.09x 10scells ml1 while minimum cell density was found in treatment T1 (Si concentration 0 uM). Lipid content was varying at different treatments and found to be maximum 25.6 ±0.48% of dry biomass in treatment T2 (Si concentration 53 uM) and minimum 14.23± 0.33 % of dry biomass in treatment T4 (Si concentration 159 uM ).Treatment T1 and T3(control), the lipid content was 20.56±0.28% and 16.74 ±0.40% of dry biomass respectively. The highest lipid content was 25.6 ±0.48% of dry biomass in treatment T2 (Si concentration 53 uM) Hence, this treatment was selected for transesterification of lipid by methanol and KOH. Subsequently, Fatty Acid Methyl Ester (FAME) analysis showed the presence of saturated fatty acids of 69.62% which is highly abundant than monounsaturated and polyunsaturated fatty acid. In the present study, the fatty acids was recorded in ranged between C16-C18, which accounts for more than 90% fatty acids being predominant components for biodiesel production making C. mue/Zer/species highly suitable forfurther exploration and commercial biodiesel production.