Evaluating the antioxidant enzymes activities, lipid peroxidation and proteomic profile changing in UCB-1 pistachio rootstock leaf under drought stress

摘要

Drought is a significant environmental stress that adversely affects plants growth and results in limiting crop yields and productivity. Also, drought stress is able to change many physiological processes in plants, especially photosynthetic capacity. To obtain a better understanding of the University of California at Berkeley I (UCB-1) pistachio rootstock in response to drought stress, some biochemical characteristics and comparative proteomic analysis were evaluated. 7-month-old UCB-1 pistachio rootstock was exposed to two different levels of drought stress for 30 days and the leaf of this rootstock was selected as experimental material. Two levels of drought stress included irrigation every 4 days with 100% field capacity as control condition and irrigation every 10 days with 50% field capacity as stress condition. Our results showed that when drought stress increased, the levels of malondialdehyde (MDA), guaiacol peroxidase (GPX), superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) were increased, significantly. Also, 18 differentially expressed proteins, which had roles in various biological processes, were identified successfully by MALDI-TOF/TOF MS. Fibroin heavy chain-like, thylakoid ltunenal 19 kDa protein, superoxide dismutase (SOD) [Cu-Zn] isoform X2 and vesicle-associated membrane protein 722-like proteins were recognized in response to drought stress, and vesicle-associated protein 2-2-like isoform X1, Golgin subfamily A member 5 and Vacuolar protein sorting-associated protein 52 A were recognized for the first time in response to abiotic stresses. Also, our results can prove that UCB-1 pistachio rootstock is a tolerant plant under drought stress condition.