The development of cold resistance rootstock using Agrobacterium-mediated transformation of Arabidopsis CBF3/DREB1A in bottle gourd (Lageneraria siceraria Standl.)

摘要

Plants growth and development are adversely affected by various abiotic stresses. Water and temperature plays important role on plant growth and crop yield. C-repeat binding factor (CBF) acts as transcription factor in plant that involve in cold-response pathway. It has major role in cold acclimation and cold response gene expression. An Agrobacterium-mediated transformation system has been established for introducing foreign gene into bottle gourd. We have introduced Arabidopsis CBF3/DREBIA gene into bottle gourd (Lageneraria siceraria Standl.) using Agrobacterium-mediated transformation. Experimental results confirmed that transgenic plants were better cold resistant than non-transgenic plants and CBF3/DREBIA gene was not transferred to stem, leaf and fruit of the grafted watermelon (Citrullus lanatus var. lanatus) from transgenic bottle gourd rootstock. Horticultural traits were similar for transgenic and non-transgenic plants. We concluded that the rootstock of transgenic bottle gourd might be used for growing watermelon at low soil temperature. (C) 2016 Elsevier B.V. All rights reserved.