A one-step incubation ELISA kit for rapid determination of dibutyl phthalate in water,beverage and liquor

摘要

A one-step incubation ELISA kit based on monoclonal antibody against dibutyl phthalate(DBP)was developed.After optimizing concentrations of coating antigen,antibody and composition of the assay buffer,an inhibition curve was plotted.The IC_(50)is 29.6 ng-mL~(-1),and the detection limit for DBP is 3.6 ng-mL~(-1).Compared with other ELISA methods,this ELISA kit had a simpler sample preparation,costed less time for detection and could detect more types of sample.The recoveries of DBP in water,beverage and liquor samples were range from 78% to 110.4%,the range of coefficient of variations is 7.7-15.3%.The cross reactivity was very low(<1%)except that for butyl benzyl phthalate(3.9%)and the di-isobutyl phthalate(12.5%).The detection results in liquor showed good correlation with those from GC-MS.All data above indicated that this kit could be used as the fast and high-throughput screening of DBP in water,beverage and liquor.