Comparative analysis of excretory-secretory proteins of nematodes Trichinella spiralis and Trichinella pseudospiralis muscle larvae

摘要

Analysis of excretory-secretory (E-S) proteins produced by muscle larvae of Trichinella spiralis strain MH 259593 and Trichinella pseudospiralis strain KU 357408 by liquid chromatography tandem mass spectrometry revealed the presence of important functional proteins in both strains: deoxyribonuclease family proteins, 5'-nucleotidases and serine proteases. The revealed proteins showed different degree of identity with proteins previously found in E-S products of Trichinella, for which immunoreactivity was shown (Bien et al., 2015), as well as with those predicted based on the results of DNA and RNA sequence analysis. The results of this work point to the importance of further investigation of the variety of E-S proteins, obtained from Trichinella domestic strains of different taxonomic origin, to find early diagnostic antigens or potential candidates for development of a polyvalent vaccine.