Impact of nano Chelated Iron, GA3 and Organic Fertilizer (Acadian) in Moringa leaves Content of a-Tocopherol and Phytosterols

摘要

Pots experiment was conducted during the summer season 2016 in the Department of Biology/College of Education/AL- Qadisiya University to study the effect of nano chelated iron, GA3 and organic fertilizer (Acadian) in the Content of a-tocopherol and Phytosterols (gamma-Sitosterol, Stigmasterol and Campesterol) in Moringa leaves. The experiment was designed as Randomized Complete Blocks (RCBD) in a factorial arrangement with three replicate. These are involved 5 concentrations of nano chelated (0, 1, 2, 3 and 4) g.l, GA3 concentrations were used (0, 200 and 400) mg.l'1 and organic fertilizer Acadian with (0,1) g.l"1. After 150 days from the date of germination, Using the GCMS device to measure the percentage a-tocopherol and Phytosterols. Means were compared by using least significant difference (LSD) at 0.05 level probability. Results showed: the concentration of 2g.L'1 of the nano-chelated iron achieved the highest percentage of Stigmasterol and Campesterol. 4 g.I/'of the nano-chelated ironsignificant decrease in the percentageof a-tocopherol. No significant effect of nano-chelated iron on the percentage of gamma-Sitosterol was found. The concentration of 200 mg.l'1 GA3 significantly increased plant sterols (gamma-Sitosterol, Stigmasterol and Campesterol). GA3 had a negative effect on the percentage of a-tocopherol, Acadian had no significant effect on the active substances studied.The three-way interaction of the studied factors showed that the highest percentage of a-tocopherol increased with the use of 2 g.Hnano-iron with 200 mg. HGA3 and 1 g.Hof Acadian fertilizer. The use of 1 g.^'nano-iron with 400 mg. 1"'GA3 and 1 g.Hof Acadian fertilizer to increase the percentage of Stigmasterol, while the combination of lg.Hnano-iron with 200 mg. 1"LGA3 and 1 g.Hof Acadian increased the percentage of Campesterol Therefore, we recommend using the combinations above to obtain the highest percentage of active substancesa-tocopherol, Stigmasterol and Campesterol due to its economic feasibility.