Ultrahigh‐performance liquid chromatography coupled with triple quadrupole and time‐of‐flight mass spectrometry for the screening and identification of the main flavonoids and their metabolites in rats after oral administration of        <fc xmlns='http://www.wiley.com/namespaces/wiley'>          <fi>Cirsium japonicum</fi>        </fc>          <fi xmlns='http://www.wiley.com/namespaces/wiley'>Cirsium japonicum</fi>Cirsium japonicum                DC. extract

Zhang Xia; Liao Man; Cheng Xiaoye; Liang Caijuan; Diao Xinpeng; Zhang Lantong

摘要

Rationale > <fc> <fi>Cirsium japonicum</fi> </fc> DC., a traditional Chinese medicine, has been shown to have anti‐haemorrhagic and anti‐tumour effects. Pharmacological studies have demonstrated that this curative effect may be related to flavonoids. The present work aimed to screen and identify the main flavonoids and their corresponding metabolites in rats after oral administration of <fc> <fi>Cirsium japonicum</fi> </fc> DC. extract. </section> <section xml:id="rcm8161-sec-0002" numbered="no"> <title type="main">Methods >A rapid and simple method based on ultrahigh‐performance liquid chromatography coupled with triple quadrupole and time‐of‐flight mass spectrometry (UHPLC/QTOF‐MS) was developed for the identification of the primary absorbing components and metabolites of the principal flavonoids. The absorbing components were first characterized, followed by the selection of representative constituents. In this study, the main flavonoids, pectolinarin, linarin and pectolinarigenin, were selected as templates to identify possible metabolites. </section> <section xml:id="rcm8161-sec-0003" numbered="no"> <title type="main">Results >A total of 27 metabolites were detected in rat blood, urine and bile samples. A hydrolysis reaction was the first step for pectolinarin and linarin, followed by oxidation and reduction reactions. However, phase II metabolites for pectolinarin and linarin were not detected. The primary biotransformation routes of pectolinarigenin were identified as oxidation, reduction, hydrolysis, and glucuronide and glucose conjugation. </section> <section </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><Abstract XMLNS =“http://www.wiley.com/namespaces/wiley”type =“main”xml：lang =“en”> <section xml：id =“rcm8161-sec-0001”编号=“否” > <标题类型=“main”>基本原理</ title> > <fc> <fi> cirsium japonic </ fi> </ fc> dc。，中药，已显示有抗出血性和抗血肿抗肿瘤效应。药理学研究表明，该疗效可能与黄酮类化有关。目前的作品旨在筛选和鉴定口服施用<FC> <FI>柳树碱</ FI> DC大鼠大鼠的主要类黄酮及其相应的代谢物。提炼。 </ p> </ section> <section xml：id =“rcm8161-sec-0002”编号=“否”> <title type =“main”>方法</ title> >基于基于的快速和简单的方法耦合与三重四极掺换和飞行时间质谱（UHPLC / QTOF-MS）的超高化学液相色谱，用于鉴定主要吸收组分和主要类黄酮的代谢物。首先表征吸收组分，然后选择代表性成分。在该研究中，选择主要的类黄酮，果皮素，临床和果皮原素作为模板以识别可能的代谢物。</ p> </ section> <section xml：id =“rcm8161-sec-0003”编号=“否”> <标题类型=“main”>结果</标题> >在大鼠血液，尿液和胆汁样品中检测总共27种代谢物。水解反应是果林素和临床的第一步，然后是氧化和还原反应。然而，未检测到果林素和临床素的II期代谢物。果皮原素素的一次生物转化途径被鉴定为氧化，还原，水解和葡糖醛酸和葡萄糖缀合物。</ p> </段> <截面 </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" > 著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-26627/'>《Rapid Communications in Mass Spectrometry: RCM 》</a> <b style="margin: 0 2px;">|</b><span>2018年第16期</span><b style="margin: 0 2px;">|</b> <span>共11页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Zhang Xia&option=202" target="_blank" rel="nofollow">Zhang Xia;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Liao Man&option=202" target="_blank" rel="nofollow">Liao Man;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Cheng Xiaoye&option=202" target="_blank" rel="nofollow">Cheng Xiaoye;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Liang Caijuan&option=202" target="_blank" rel="nofollow">Liang Caijuan;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Diao Xinpeng&option=202" target="_blank" rel="nofollow">Diao Xinpeng;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Zhang Lantong&option=202" target="_blank" rel="nofollow">Zhang Lantong;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>Department of Pharmaceutical Analysis School of PharmacyHebei Medical UniversityShijiazhuang China;</p> <p>Department of Pharmaceutical Analysis School of PharmacyHebei Medical UniversityShijiazhuang China;</p> <p>Department of Pharmaceutical Analysis School of PharmacyHebei Medical UniversityShijiazhuang China;</p> <p>Department of Pharmaceutical Analysis School of PharmacyHebei Medical UniversityShijiazhuang China;</p> <p>Department of Pharmaceutical Analysis School of PharmacyHebei Medical UniversityShijiazhuang China;</p> <p>Department of Pharmaceutical Analysis School of PharmacyHebei Medical UniversityShijiazhuang China;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li> <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span> <a href="https://www.zhangqiaokeyan.com/clc/1187.html" title="分析化学">分析化学 ;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> </p> </li> </ul> </div> </div> <div class="literature cardcommon"> <div class="similarity "> <h3 class="all_title" id="enpatent66">相似文献</h3> <div class="similaritytab clearfix"> <ul> <li class="active" >外文文献</li> </ul> </div> <div class="similarity_details"> <ul > <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704024020574.html">Ultrahigh‐performance liquid chromatography coupled with triple quadrupole and time‐of‐flight mass spectrometry for the screening and identification of the main flavonoids and their metabolites in rats after oral administration of <fc xmlns="http://www.wiley.com/namespaces/wiley"> <fi>Cirsium japonicum</fi> </fc> <fi xmlns="http://www.wiley.com/namespaces/wiley">Cirsium japonicum</fi>Cirsium japonicum DC. extract</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Zhang 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