Optimization of sample preparation for intact cell mass spectrometry (matrix‐assisted laser desorption/ionization linear time‐of‐flight mass spectrometry) of endophytic <i xmlns='http://www.wiley.com/namespaces/wiley'>XylariaXylaria

Silva Amaral Luciana; Rodrigues‐Filho Edson; Kubicek Christian P.; Herwig Christoph; Marchetti‐Deschmann Martina; Allmaier Günter

摘要

Rationale > Although the fruiting‐body of the fungi of the genus <fi>Xylaria</fi> shows a great variety of morphological characteristics, their mycelial forms are always very similar, imposing difficulties for their identification. Intact cell mass spectrometry (ICMS) using matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry (MALDI‐TOFMS) can be a fast and reliable strategy to support the differentiation/identification of <fi>Xylaria</fi> species in those cases where fruit‐bodies are not available. </section> <section xml:id="rcm8098-sec-0002" numbered="no"> <title type="main">Methods > Many experimental parameters such as sample preparation and culture media are crucial for filamentous fungi analysis by MALDI‐TOFMS. For the purposes of this study, we used four matrices (CHCA, DHB, FA and SA) with five different concentrations (0.1, 0.3, 0.5, 1.0 and 2.5%) of TFA in the matrix, the influence of six different culture media (solid and liquid), and three mycelium peptide/protein extraction protocols (acid, basic and thymol‐supported solution) to optimize the sample preparation of the endophytic fungus <fi>X. arbuscula</fi> . </section> <section xml:id="rcm8098-sec-0003" numbered="no"> <title type="main">Results > It was observed that sinapinic acid (30?mg/mL) dissolved in acetonitrile/0.1% TFA and PDA were the best matrix solution and culture medium, respectively, for the ICMS of <fi>X. arbuscula</fi> . The formic acid and ammonium bicarbonate (AB) protocols provided similar mass spectra; however, a higher number of peaks we </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><Abstract XMLNS =“http://www.wiley.com/namespaces/wiley”type =“main”xml：lang =“en”> <section xml：id =“rcm8098-sec-0001”编号=“否” > <标题类型=“main”>理由</ title> >虽然Genus的真菌的果实<fi>木耳</ fi>显示出各种形态特征，但它们的菌丝形式总是非常类似，对他们的身份造成困难。使用基质辅助激光解吸/电离飞行时间质谱法（MALDI-TOFM）的完整细胞质谱（ICMS）可以是一种快速可靠的策略，以支持<FI>木耳的分化/鉴定物种在那些果实不可用的情况下。 </ p> </ section> <第XML：ID =“RCM8098-SEC-0002”编号=“否”> <标题类型=“main”>方法</ title> >许多实验参数，如样品制备培养介质对Maldi-Tofms进行丝状真菌分析至关重要。出于本研究的目的，我们在基质中使用了四种不同浓度（0.1,0.3,0.5,1.0和2.5％）TFA的矩阵（0.1,0.3,0.5,1.0和2.5％），影响六种不同的培养基（固体和液体）和三种菌丝肽/蛋白质提取方案（酸，碱性和胸腺和胸蛋白溶液），以优化内生真菌的样品制备<FI> x。 arbuscula </ fi>。 </ p> </ section> <section XML：ID =“RCM8098-SEC-0003”编号=“否”> <标题类型=“main”>结果</标题> >它被观察到锡丁酸（溶解在乙腈/ 0.1％TFA和PDA中的30×mg / ml）分别用于<FI> x的ICMS的最佳基质溶液和培养基。 arbuscula </ fi>。甲酸和碳酸氢铵（AB）方案提供了类似的质谱;但是，我们的峰值较多 </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" > 著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-26627/'>《Rapid Communications in Mass Spectrometry: RCM 》</a> <b style="margin: 0 2px;">|</b><span>2018年第10期</span><b style="margin: 0 2px;">|</b> <span>共9页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Silva Amaral Luciana&option=202" target="_blank" rel="nofollow">Silva Amaral Luciana;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Rodrigues‐Filho Edson&option=202" target="_blank" rel="nofollow">Rodrigues‐Filho Edson;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Kubicek Christian P.&option=202" target="_blank" rel="nofollow">Kubicek Christian P.;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Herwig Christoph&option=202" target="_blank" rel="nofollow">Herwig Christoph;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Marchetti‐Deschmann Martina&option=202" target="_blank" rel="nofollow">Marchetti‐Deschmann Martina;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Allmaier Günter&option=202" target="_blank" rel="nofollow">Allmaier Günter;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>Departamento de QuímicaUniversidade Federal de S?o Carlos CP 67613 565‐905 S?o Carlos SP Brazil;</p> <p>Departamento de QuímicaUniversidade Federal de S?o Carlos CP 67613 565‐905 S?o Carlos SP Brazil;</p> <p>Institute of Chemical Environmental and Bioscience EngineeringTU Wien (Vienna University of Technology)Getreidemarkt 9/166 A‐1060 Vienna Austria;</p> <p>Institute of Chemical Environmental and Bioscience EngineeringTU Wien (Vienna University of Technology)Getreidemarkt 9/166 A‐1060 Vienna Austria;</p> <p>Institute of Chemical Technologies and AnalyticsTU Wien (Vienna University of Technology)Getreidemarkt 9/164 A‐1060 Vienna Austria;</p> <p>Institute of Chemical Technologies and AnalyticsTU Wien (Vienna University of Technology)Getreidemarkt 9/164 A‐1060 Vienna Austria;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li> <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span> <a href="https://www.zhangqiaokeyan.com/clc/1187.html" title="分析化学">分析化学 ;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> </p> </li> </ul> </div> </div> <div class="literature cardcommon"> <div class="similarity "> <h3 class="all_title" id="enpatent66">相似文献</h3> <div class="similaritytab clearfix"> <ul> <li class="active" >外文文献</li> <li >专利</li> </ul> </div> <div class="similarity_details"> <ul > <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704024020505.html">Optimization of sample preparation for intact cell mass spectrometry (matrix‐assisted laser desorption/ionization linear time‐of‐flight mass spectrometry) of endophytic <i xmlns="http://www.wiley.com/namespaces/wiley">XylariaXylaria</a> <b>[J]</b> . <span> 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Optimization of sample preparation for intact cell mass spectrometry (matrix‐assisted laser desorption/ionization linear time‐of‐flight mass spectrometry) of endophytic XylariaXylaria

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