Rapid detection of severe fever with thrombocytopenia syndrome virus (SFTSV) total antibodies by up‐converting phosphor technology‐based lateral‐flow assay

机译：快速检测血小板减少症综合征病毒（SFTSV）总抗体通过加上磷光体技术的侧向流动测定

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

Abstract >In this study, an up‐converting phosphor technology‐based lateral‐flow (UPT‐LF) assay was developed to detect severe fever with thrombocytopenia syndrome virus (SFTSV) total antibodies rapidly and specifically. SFTSV recombinant N protein (SFTSV‐rNP) was coated on analytical membrane for sample capture, up‐converting phosphor (UCP) particles were used as the reporter, the luminescence emitted by UCP particles was converted to a measurable signal by a biosensor. The performance of UPT‐LF assay was evaluated by testing 302 field serum samples by ELISA (enzyme‐linked immunosorbent assay), Western blotting and UPT‐LF assay. UPT‐LF assay exhibited a lower detection limit than ELISA, and a satisfied level of agreement was exhibited by Kappa statistics (Kappa coefficient?=?0.938). Considering Western blotting as the reference for comparison, the sensitivity and specificity of UPT‐LF assay could reach 98.31% and 100%. UPT‐LF assay showed no specific reaction with hantavirus total serum antibodies, which avoids the misdiagnosis of SFTSV from hantavirus that could cause similar clinical symptoms. UPT‐LF assay was able to achieve acceptable results within 15?min and needed only 10?μL sample for each test. As a whole, UPT‐LF assay is a candidate method for on‐site surveillance of SFTSV total antibodies owing to its excellent sensitivity, specificity, stability, easy operation and for being less time consuming. </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><Abstract Type =“main”XML：Lang =“en”> <标题类型=“main”>抽象</ title> >在本研究中，一个加换磷光体技术的横向流量（UPT-LF ）开发的测定以迅速且特别是抗血小阴盆综合征病毒（SFTSV）总抗体来检测严重发烧。将SFTSV重组N蛋白（SFTSV-RNP）涂覆在用于样品捕获的分析膜上，使用上转换磷光体（UCP）颗粒作为报告，通过生物传感器将由UCP颗粒发出的发光转化为可测量的信号。通过通过ELISA（酶联免疫吸附测定），Western印迹和UPT-LF测定来测试302个现场血清样品来评估UPT-LF测定的性能。 UPT-LF测定表现出比ELISA更低的检测限，Kappa统计（Kappa系数？= 0.938）展出了满足的协议水平。考虑Western印迹作为比较的参考，UPT-LF测定的敏感性和特异性可达到98.31％和100％。 UPT-LF测定显示出与Hantavirus总血清抗体的特异性反应，这避免了汉坦病毒的SFTSV误诊，这可能导致类似的临床症状。 UPT-LF测定能够在15？min内获得可接受的结果，并且每次测试只需要10μl样品。作为一个整体，UPT-LF测定是由于其优异的灵敏度，特异性，稳定性，易于操作以及较少耗时的较好的SFTSV总抗体的候选方法。</ p> </摘要> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-26441/'>《Luminescence: The journal of biological and chemical luminescence》</a> <b style="margin: 0 2px;">|</b><span>2019年第2期</span><b style="margin: 0 2px;">|</b><span>共6页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Huang Chao&option=202" target="_blank" rel="nofollow">Huang Chao;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Wei Qiaozhen&option=202" target="_blank" rel="nofollow">Wei Qiaozhen;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Hu Qiushi&option=202" target="_blank" rel="nofollow">Hu Qiushi;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Wen Tian&option=202" target="_blank" rel="nofollow">Wen Tian;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Xue Lei&option=202" target="_blank" rel="nofollow">Xue Lei;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Li Shuang&option=202" target="_blank" rel="nofollow">Li Shuang;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Zeng Xiaoyan&option=202" target="_blank" rel="nofollow">Zeng Xiaoyan;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Shi Fengjuan&option=202" target="_blank" rel="nofollow">Shi Fengjuan;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Jiao Yongjun&option=202" target="_blank" rel="nofollow">Jiao Yongjun;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Zhou Lei&option=202" target="_blank" rel="nofollow">Zhou Lei;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>Institute of Pathogenic Microbiology Jiangsu Provincial Center for Disease Prevention and ControlNanjing China;</p> <p>School of Laboratory Medicine and Life SciencesWenzhou Medical UniversityWenzhou China;</p> <p>National Key Laboratory of Biochemical Engineering PLA Key Laboratory of Biopharmaceutical Production &</p> <p>Formulation EngineeringInstitute of Process Engineering Chinese Academy of SciencesBeijing China;</p> <p>Institute of Pathogenic Microbiology Jiangsu Provincial Center for Disease Prevention and ControlNanjing China;</p> <p>National Key Laboratory of Biochemical Engineering PLA Key Laboratory of Biopharmaceutical Production &</p> <p>Formulation EngineeringInstitute of Process Engineering Chinese Academy of SciencesBeijing China;</p> <p>National Key Laboratory of Biochemical Engineering PLA Key Laboratory of Biopharmaceutical Production &</p> <p>Formulation EngineeringInstitute of Process Engineering Chinese Academy of SciencesBeijing China;</p> <p>Institute of Pathogenic Microbiology Jiangsu Provincial Center for Disease Prevention and ControlNanjing China;</p> <p>Institute of Pathogenic Microbiology Jiangsu Provincial Center for Disease Prevention and ControlNanjing China;</p> <p>Institute of Pathogenic Microbiology Jiangsu Provincial Center for Disease Prevention and ControlNanjing China;</p> <p>National Key Laboratory of Biochemical Engineering PLA Key Laboratory of Biopharmaceutical Production &</p> <p>Formulation EngineeringInstitute of Process Engineering Chinese Academy of SciencesBeijing China;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/1345.html" title="生物光学">生物光学;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=rapid detection&option=203" rel="nofollow">rapid detection;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=severe fever with thrombocytopenia syndrome virus&option=203" rel="nofollow">severe fever with thrombocytopenia syndrome virus;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=up‐converting phosphor technology‐based lateral‐flow assay&option=203" rel="nofollow">up‐converting phosphor technology‐based lateral‐flow assay;</a> </p> <div class="translation"> 机译：快速检测;严重发烧血小板减少症综合征病毒;上转换磷光体技术的侧向流动测定; </div> </li> </ul> </div> </div> <div class="literature cardcommon"> <div class="similarity "> <h3 class="all_title" id="enpatent66">相似文献</h3> <div class="similaritytab clearfix"> <ul> <li class="active" >外文文献</li> <li >中文文献</li> <li >专利</li> </ul> </div> <div class="similarity_details"> <ul > <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704023986339.html">Rapid detection of severe fever with thrombocytopenia syndrome virus (SFTSV) total antibodies by up‐converting phosphor technology‐based lateral‐flow assay</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Huang Chao&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Huang Chao,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Wei Qiaozhen&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Wei Qiaozhen,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Hu Qiushi&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Hu Qiushi,</a> <a href="/journal-foreign-26441/" target="_blank" rel="nofollow" class="tuijian_authcolor">Luminescence: The journal of biological and chemical luminescence .</a> <span>2019</span><span>,第2期</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：快速检测血小板减少症综合征病毒（SFTSV）总抗体通过加上磷光体技术的侧向流动测定</span> </p> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/academic-journal-foreign_detail_thesis/0204114681139.html">Ultrasensitive Detection of Severe Fever with Thrombocytopenia Syndrome Virus Based on Immunofluorescent Carbon Dots/SiO2 Nanosphere-Based Lateral Flow Assay</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Lai-Di Xu&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Lai-Di Xu,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Qing Zhang&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Qing Zhang,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Shou-Nian Ding&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Shou-Nian Ding,</a> <a href="/journal-foreign-18130/" target="_blank" rel="nofollow" class="tuijian_authcolor">ACS Omega .</a> <span>2019</span><span>,第25期</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：基于免疫荧光碳点/ SiO2纳米球的侧向流测定法对血小板减少综合征病毒的高热进行超灵敏检测</span> </p> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704013212200.html">Development of up-converting phosphor technology-based lateral-flow assay for rapidly quantitative detection of hepatitis B surface antibody.</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Li L&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Li L,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Zhou L&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Zhou L,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Yu Y&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Yu Y,</a> <a href="/journal-foreign-33023/" target="_blank" rel="nofollow" class="tuijian_authcolor">Diagnostic microbiology and infectious disease .</a> <span>2009</span><span>,第2期</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：基于上转换荧光粉技术的侧向流分析技术的开发，可快速定量检测乙型肝炎表面抗体。</span> </p> </li> <li> <div> <b>4. </b><a class="enjiyixqcontent" href="/academic-conference-foreign_meeting-12028_thesis/0705014372950.html">Portable image-based detection system for Upconverting phosphor immune lateral flow assay</a> <b>[C]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Chao Li&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Chao Li,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Meng Chen&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Meng Chen,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Zhi Cheng&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Zhi Cheng,</a> <a href="/conference-foreign-12028/" target="_blank" rel="nofollow" class="tuijian_authcolor">International Conference on Biological Information and Biomedical Engineering .</a> <span>2019</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：基于便携式图像的检测系统，用于上变荧光体免疫横向流动测定</span> </p> </li> <li> <div> <b>5. </b><a class="enjiyixqcontent" href="/academic-degree-foreign_mphd_thesis/02061271976.html">Development and validation of a blocking enzyme-linked immunosorbent assay for the detection of antibodies against porcine reproductive and respiratory syndrome virus.</a> <b>[D] </b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Ferrin, Neal Harold.&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Ferrin, Neal Harold. </a> <span>2004</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：开发和验证用于检测针对猪繁殖与呼吸综合征病毒的抗体的封闭酶联免疫吸附法。</span> </p> </li> <li> <div> <b>6. </b><a class="enjiyixqcontent" href="/academic-journal-foreign-pmc_detail_thesis/040007217805.html">An up-converting phosphor technology-based lateral flow assay for rapid detection of major mycotoxins in feed: Comparison with enzyme-linked immunosorbent assay and high-performance liquid chromatography-tandem mass spectrometry</a> <b>[O] </b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Fenghua Zhu&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Fenghua Zhu,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Beibei Zhang&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Beibei Zhang,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Lianqin Zhu&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Lianqin Zhu </a> <span>2021</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：基于磷光体技术的侧向流动测定用于快速检测进料中的主要霉菌毒素：与酶联免疫吸附测定和高效液相色谱 - 串联质谱法比较</span> </p> </li> <li> <div> <b>7. </b><a class="enjiyixqcontent" href="/open-access_resources_thesis/01000127693227.html">An up-converting phosphor technology-based lateral flow assay for rapid detection of major mycotoxins in feed: Comparison with enzyme-linked immunosorbent assay and high-performance liquid chromatography-tandem mass spectrometry</a> <b>[O] </b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Fenghua Zhu&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Fenghua Zhu,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Beibei Zhang&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Beibei Zhang,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Lianqin Zhu&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Lianqin Zhu </a> <span>2021</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：基于磷光体技术的侧向流动测定，用于快速检测进料中的主要霉菌毒素：与酶联免疫吸附测定和高效液相色谱 - 串联质谱法比较</span> </p> </li> <li> <div> <b>8. </b><a class="enjiyixqcontent" href="/ntis-science-report_ad_thesis/02071367304.html">Rapid Active Assay for the Detection of Antibodies to West Nile Virus in Chickens.</a> <b>[R] </b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Groves, S. S.&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Groves, S. S.,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext= Turell, M. J.&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> Turell, M. J.,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext= Bailey, C. L.&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> Bailey, C. L.,</a> <span>2008</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：用于检测鸡中西尼罗河病毒抗体的快速活性测定法。</span> </p> </li> </ul> <ul style="display: none;"> <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/academic-journal-cn_veterinary-orientation_thesis/0201260726396.html">严重发热伴血小板减少综合征病毒(SFTSV)总抗体检测试剂盒(ELISA)的研制</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=马玉英&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 马玉英</a> <span> <a href="/journal-cn-7067/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 兽医导刊 </a> </span> <span> . 2016</span><span>,第010期</span> </span> </div> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/academic-journal-cn_henan-journal-animal-husbandry-veterinary-medicine_thesis/0201260832202.html">严重发热伴血小板减少综合征病毒(SFTSV)总抗体检测试剂盒(ELISA)的研制</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=马玉英&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 马玉英</a> <span> <a href="/journal-cn-7098/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 河南畜牧兽医（综合版） </a> </span> <span> . 2016</span><span>,第007期</span> </span> </div> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/academic-journal-cn_shenyang-force-medicine_thesis/0201267094556.html">微量法快速检测肾综合征出血热病毒特异性抗体的自身血凝试验</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=董萍&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 董萍</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=曲菲&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,曲菲</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=等&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,等</a> <span> <a href="/journal-cn-40245/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 沈阳部队医药 </a> </span> <span> . 2002</span><span>,第001期</span> </span> </div> </li> <li> <div> <b>4. </b><a class="enjiyixqcontent" href="/academic-journal-cn_monoclonal-antibody-communication_thesis/0201293411760.html">反向间接血凝抑制试验检测不同种属动物血清中肾综合征血热病毒特异性总抗体的探讨</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=唐家琪&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 唐家琪</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=李先富&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,李先富</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=陈竞芳&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,陈竞芳</a> <span> <a href="/journal-cn-51997/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 单克隆抗体通讯 </a> </span> <span> . 1989</span><span>,第2期</span> </span> </div> </li> <li> <div> <b>5. </b><a class="enjiyixqcontent" href="/academic-journal-cn_detail_thesis/0201293403061.html">运用免疫胶体金技术快速检测丙型肝炎病毒抗体</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=蒋树海&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 蒋树海</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=杨发青&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,杨发青</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=LEE Tony&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,LEE Tony</a> <span> <a href="/journal-cn-56801/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 解放军预防医学杂志 </a> </span> <span> . 2009</span><span>,第1期</span> </span> </div> </li> <li> <div> <b>6. </b><a class="enjiyixqcontent" href="/academic-conference-cn_meeting-10739_thesis/02022195386.html">乙型肝炎病毒表面抗体(乙肝疫苗保护性抗体)快速测定方法的建立和应用评价</a> <b>[C]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=范黎&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 范黎</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=陈霞&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,陈霞</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=王俊&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,王俊</a> <span> <a href="/conference-cn-10739/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 第五次全国免疫诊断暨疫苗学术研讨会 </a> <span> <span> . 2011</span> </span> </div> </li> <li> <div> <b>7. </b><a class="enjiyixqcontent" href="/academic-degree-domestic_mphd_thesis/020311308108.html">SELEX技术筛选弓形虫与巨细胞病毒抗体适配子及其SPR传感器快速检测抗体的方法学研究</a> <b>[A] </b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=刘星&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 刘星</a> <span> . 2012</span> </span> </div> </li> </ul> <ul style="display: none;"> <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/patent-detail/06120211148251.html">一种基于上转化发光纳米材料的SFTSV总抗体免疫层析快速检测试纸条</a> <b>[P]</b> . <span> 中国专利： CN211148668U </span> <span> . 2020.07.31</span> </div> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/patent-detail/061203025937.html">RT-RPA与侧向流动层析技术相结合的猪嵴病毒快速检测方法及其检测试剂盒</a> <b>[P]</b> . <span> 中国专利： CN107541571B </span> <span> . 2018.09.28</span> </div> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/patent-detail/06130411502820.html">PRODUCTION AND APPLICATION OF A MONOCLONAL ANTIBODY AND THE DEVELOPMENT OF A COMPETITIVE ENZYME-LINKED IMMUNOSORBENT ASSAY(C-ELISA) FOR DETECTION OF SEVERE FEVER WITH THROMBOCYTOPENIA SYNDROME VIRUS(SFTSV)</a> <b>[P]</b> . <span> 外国专利：  KR20160054161A </span> <span> . 2016-05-16</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：单克隆抗体的生产和应用以及竞争性酶联免疫吸附法（C-ELISA）的检测以血小板减少症综合征病毒（SFTSV）检测重度发热 </span> </p> </li> <li> <div> <b>4. </b><a class="enjiyixqcontent" href="/patent-detail/06130402395162.html">KIT FOR DETECTING ANTIBODY OF SEVERE FEVER WITH THROMBOCYTOPENIA SYNDROME VIRUS AND METHOD FOR DETECTING ANTIBODY OF SEVERE FEVER WITH THROMBOCYTOPENIA SYNDROME VIRUS</a> <b>[P]</b> . <span> 外国专利：  JP2020026953A </span> <span> . 2020-02-20</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：血小板减少症综合征病毒检测重度抗体的试剂盒和血小板减少症综合征病毒检测重度抗体的方法 </span> </p> </li> <li> <div> <b>5. </b><a class="enjiyixqcontent" href="/patent-detail/06130499533257.html">COMPOSITION FOR DETECTING SEVERE FEVER WITH THROMBOCYTOPENIA SYNDROME VIRUS GENE AND METHOD FOR DIAGNOSING SEVERE FEVER WITH THROMBOCYTOPENIA SYNDROME USING SAME</a> <b>[P]</b> . <span> 外国专利：  WO2021040245A1 </span> <span> . 2021-03-04</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：用血小板减少综合征病毒基因检测严重发烧的组合物及其使用方法对血小板减少症综合征进行严重发烧的方法 </span> </p> </li> </ul> </div> </div> </div> <div class="theme cardcommon" style="overflow: auto;display:none"> <h3 class="all_title" id="enpatent55">相关主题</h3> <ul id="subject"> </ul> </div> </div> </div> </div> <div class="right rightcon"> <div class="details_img cardcommon clearfix" style="margin-bottom: 10px;display:none;" > </div> </div> </div> <div id="thesis_get_original1" class="downloadBth" style="bottom: 19px;z-index: 999;" onclick="ywcd('0704023986339','4',7,2,1,'',this,24)" class="delivery" prompt="010401" title="通过人工服务将文献原文发送至邮箱" >获取原文</div> <div class="journalsub-pop-up" style="display: none"> <div class="journal-sub"> <h2>期刊订阅</h2> <img src="https://cdn.zhangqiaokeyan.com/img/loginclose.png" alt="" onclick="$('.journalsub-pop-up').hide()"> <p class="pardon">抱歉，该期刊暂不可订阅，敬请期待！</p> <p class="current">目前支持订阅全部北京大学中文核心（2020）期刊目录。</p> <div style="display: flex;margin-top: 69px;justify-content: space-between;"> <div class="no-sub" onclick="$('.journalsub-pop-up').hide()">暂不订阅</div> <div class="other-sub" onclick="continueSub('from=pc-detail')">继续订阅其他期刊</div> </div> </div> </div> <div class="right_btn"> <ul> <li class="gouwuche">  </li> <li class="yijian"> <a href="javascript:void(0);" onclick="link_analysis('/mycenter/auth/complaint.html',this)">意见反馈</a> </li> <li class="top"> <a href="javascript:scrollTo(0,0);">回到顶部</a> </li> <li class="shouye"> <a href="/">回到首页</a> </li> </ul> </div> <div class="xllindexfooter"> <div class="xllindexfootercenter"> <div class="xllindexfooterleft left" > <div class="xllindexfooterleftli"> <ul> <li><a href="/about.html">关于掌桥</a></li> <li><a href="/help/helpmap.html">资源导航</a></li> <li><a href="/help/helpguide.html">新手指南</a></li> <li><a href="/help/helpcenter.html">常见问题</a></li> <li><a href="/sitemap.html">网站地图</a></li> <li><a href="/help/helpcenter.html?type=9">版权声明</a></li> </ul> </div> <div class="xllindexfooterleft"> <p class="xllindexfooterlefteamil">客服邮箱：kefu@zhangqiaokeyan.com</p> <div class="xllindexfooterlefttcp"> <div class="xllindexfooterpoliceiimg"></div> <div class="xllindexfooterpoliceispan"> <span>京公网安备：11010802029741号 </span> <span>ICP备案号：<a href="https://beian.miit.gov.cn" rel="nofollow" target="_blank">京ICP备15016152号-6</a></span> <span>六维联合信息科技 (北京) 有限公司©版权所有</span> </div> </div> </div> </div> <div class="xllindexfooterright left"> <ul> <li> <p style="font-weight: bold;">客服微信</p> <div></div> </li> <li> <p style="font-weight: bold;">服务号</p> <div></div> </li> </ul> </div> </div> </div> <span id="0704023986339down" data-source="7," data-out-id="t4TZ43nArirdNlZx1c7UhGcoVbovEMlmldW8USMFGDDP3vewoMO38mHnf1B3Erfo," data-f-source-id="7" data-title="Rapid detection of severe fever with thrombocytopenia syndrome virus (SFTSV) total antibodies by up‐converting phosphor technology‐based lateral‐flow assay" data-price="20" data-site-name="" data-transnum="24" style="display:none;"></span> <input type="hidden" value="4" id="sourcetype"> <input type="hidden" value="26441" id="journalid"> <input type="hidden" value="1" id="inyn_provide_service_level"> <input type="hidden" value="https://cdn.zhangqiaokeyan.com" id="imgcdn"> <input type="hidden" value="1" id="isdeatail"> <input type="hidden" value="" id="syyn_indexed_database"> <input type="hidden" value="" id="servicetype"> <input type="hidden" id="pagename" value="Rapid detection of severe fever with thrombocytopenia syndrome virus (SFTSV) total antibodies by up‐converting phosphor technology‐based lateral‐flow assay"/> <input type="hidden" value="thesis_get_original" id="pageIdentification"> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/jquery-1.12.4.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/lwlh_ajax.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/zq.js?v=5.7.7"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/common.js?v=5.7.7"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/jquery.cookie.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/top.js?v=5.7.7"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/tip.js?v=5.7.7"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/login.js?v=5.7.7"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/down.js?v=5.7.7"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/search.js?v=5.7.7"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/searchtype.js?v=5.7.7"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/user/regist.js?v=5.7.7"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/zxs_solor/detail.js?v=5.7.7"></script> <script type="text/javascript" src="https://www.zhangqiaokeyan.com/statistics/static/pagecollection.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/tj.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/pushbaidu.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/history.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/util/cookie.js?v=5.7.7"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/weipu/weipu.js?v=5.7.7"></script> </body> <script> $(function(){ var weiPuStatus = getCookie('WeiPuStatus'); if(weiPuStatus){ tipWeiPuStatus(weiPuStatus); delCookie('WeiPuStatus'); } getFacetKeywordVoInId(); var sourcetype = $("#sourcetype").val(); var inyn_provide_service_level = $("#inyn_provide_service_level").val(); if((sourcetype ==1||sourcetype==4)&&inyn_provide_service_level!=4){ getJournal(); } }) </script> </html>