Circ RNA RNA wrap – a flexible pipeline for circ RNA RNA identification, transcript prediction, and abundance estimation

摘要

Circular RNA (circ RNA ) is a recently described RNA molecule formed by backsplicing, with the 3′ and 5′ ends linked covalently. The study of circ RNA s using RNA ‐Seq data enables the analysis of genome‐wide endogenous circular transcripts. Although several algorithms for circ RNA analysis have been developed, they have thus far had limited application. Additionally, approaches for prediction and validation of the composition of exons in circ RNA s are still being developed. In this study, we measured the effectiveness of existing tools on collected and simulated data. Furthermore, we carried out a wide‐ranging study of circ RNA workflows, which we named circ RNA wrap. Application of circ RNA wrap on the included datasets reveals that our novel protocol can assist researchers in the study of circ RNA biology.