Zinc doping induced differences in the surface composition, surface morphology and osteogenesis performance of the calcium phosphate cement hydration products

摘要

In order to investigate the influence of Zn on the hydration reaction of calcium phosphate cement (CPC), the incompletely hydrated CPC tablets were kept soaking in varying zinc-containing tris-(hydroxymethyl)-aminomethane/hydrochloric acid (Zn-Tris-HCl) buffers. It was found that Zn could retard the CPC hydration, the inhibitory effect was in direct proportional to the Zn content in the Zn-Tris-HCl buffer, and overhigh concentration of Zn (>= 800 mu M) caused the CPC hydration products having different phase composition and surface morphology. Cell culture experimental results revealed the CPC tablets which were soaked in the Zn-Tris-HCl buffer containing relative low Zn content (<= 320 mu M) favored the mouse bone mesenchymal stem cells (mBMSCs) spreading. When Zn-doped CPC tablets released 10.91 to 27.15 mu M of zinc ions into the cell culture medium, it greatly contributed to the improvement of the proliferation ability and the alkaline phosphatase (ALP) activity of the mBMSCs. In the same case, the expression of osteogenesis related genes such as collagen I and runt-related transcription factor 2 was remarkably up-regulated as well. However, the release of high concentration of Zn (128.58 mu M) would significantly reduce the ALP activity of the mBMSCs. Therefore, Zn not only facilitates osteogenesis but also affects the CPC hydration behavior, and the CPC with suitable Zn dosage concentration has great potentials to be used for clinical bone repairing.